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Journal article
Phylogenetic Lineage and Pilus Protein Spb1/SAN1518 Affect Opsonin-Independent Phagocytosis and Intracellular Survival of Group B Streptococcus
Microbes and infection, v 13(4), pp 369-382
14 Jan 2011
PMCID: PMC4500112
PMID: 21238599
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Opsonin-independent phagocytosis of Group B Streptococcus (GBS) is important in defense against neonatal GBS infections. A recent study indicated a role for GBS pilus in macrophage phagocytosis. We studied 163 isolates from different phylogenetic backgrounds and those possessing or lacking the gene encoding the pilus backbone protein, Spb1 (SAN1518, PI-2b) and
spb1
-deficient mutants of wild-type (WT) serotype III-3 GBS 874391 in non-opsonic phagocytosis assays using J774A.1 macrophages. Numbers of GBS phagocytosed differed up to 23-fold depending on phylogenetic background; isolates possessing
spb1
were phagocytosed more than isolates lacking
spb1
. Comparing WT GBS and isogenic
spb1
-deficient mutants showed WT was phagocytosed better compared to mutants; Spb1 also enhanced intracellular survival as mutants were killed more efficiently. Complementation of mutants restored phagocytosis and resistance to killing in J774A.1 macrophages. Spb1 antiserum revealed surface expression in WT GBS and spatial distribution relative to capsular polysaccharide.
spb1
did not affect macrophage nitric oxide and TNF-alpha responses; differences in phagocytosis did not correlate with N-acetyl D-glucosamine (from GBS cell wall) according to enzyme-linked lectin-sorbent assay. Together, these findings support a role for phylogenetic lineage and Spb1 in opsonin-independent phagocytosis and intracellular survival of GBS in J774A.1 macrophages.
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Details
- Title
- Phylogenetic Lineage and Pilus Protein Spb1/SAN1518 Affect Opsonin-Independent Phagocytosis and Intracellular Survival of Group B Streptococcus
- Creators
- Debasish Chattopadhyay - University of Alabama at BirminghamAlison J. Carey - Griffith UniversityElise Caliot - Institut PasteurRichard I. Webb - University of QueenslandJames R. Layton - University of Alabama at BirminghamYan Wang - St. Jude Children's Research HospitalJohn F. Bohnsack - University of UtahElisabeth E. Adderson - St. Jude Children's Research HospitalGlen C. Ulett - Griffith University
- Publication Details
- Microbes and infection, v 13(4), pp 369-382
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics; College of Medicine; Drexel University
- Web of Science ID
- WOS:000289186600009
- Scopus ID
- 2-s2.0-79952453865
- Other Identifier
- 991020099054604721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Immunology
- Infectious Diseases
- Microbiology