Journal article
Physiological validation of cell health upon probing with carbon nanotube endoscope and its benefit for single-cell interrogation
Nanomedicine, v 8(5), pp 590-598
Jul 2012
PMID: 21889477
Abstract
New-generation nanoscale devices for single-cell study are intensively being developed. As has been shown, nanodevices are minimally invasive because of their order-of-magnitude smaller size in comparison to conventional glass pipettes. However, in most studies the evaluation of the nanodevice impact on cell health has not extended to their effects on cell metabolic integrity. In this work we evaluated the degree to which the insertion of a carbon-based nanotube endoscope into a cell induces mechanical and biochemical stress, and affects cellular key metabolic systems. The effects of insertion of the nanotube endoscope on cell morphological and physiological modulations were monitored and compared to those of glass micropipettes. We report that nanotube endoscope insertion does not significantly modulate the plasma membrane and actin network. The cell metabolic mechanisms such as energy production and inositol 1,4,5-trisphosphate–dependent calcium signaling remain preserved for prolonged endoscope presence within a cell.
In this basic science study, the effects of insertion of carbon nanotube endoscope on cell morphological and physiological modulations were monitored and compared to those of glass micropipettes. Nanotube endoscope insertion is truly minimally invasive: it does not significantly modulate the plasma membrane and actin network; the energy production and inositol 1,4,5-trisphosphate–dependent calcium signaling also remain preserved during prolonged endoscope presence within a cell.
The carbon nanotube–based endoscope with cylindrical, hollow geometry and flexible, conductive tips enables intracellular probing along with fluid transfer to and from single living cells. In this work we demonstrate that the insertion of probe tips into a cell body does not induce significant mechanical stress or affect cellular key metabolic systems in terms of their structural and functional integrity. Particularly, it is shown that the nanotube endoscope insertion does not significantly modulate the plasma membrane and actin network. The cell metabolic mechanisms such as energy production and IP3-dependent calcium signaling remain preserved for prolonged endoscope presence within a cell. The carbon nanotube endoscope opens a new realm of opportunities for single-cell and single-organelle machinery study, as well as for monitoring local communications between organelles in dynamics. [Display omitted]
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Details
- Title
- Physiological validation of cell health upon probing with carbon nanotube endoscope and its benefit for single-cell interrogation
- Creators
- Zulfiya Orynbayeva - Department of Surgery, Drexel University College of Medicine, Philadelphia, Pennsylvania, USARiju Singhal - Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania, USAElina A Vitol - Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania, USAMichael G Schrlau - Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania, USAElizabeth Papazoglou - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, Pennsylvania, USAGary Friedman - Department of Electrical and Computer Engineering and A.J. Drexel Nanotechnology Institute, Drexel University, Philadelphia, Pennsylvania, USAYury Gogotsi - Department of Materials Science and Engineering, Drexel University, Philadelphia, Pennsylvania, USA
- Publication Details
- Nanomedicine, v 8(5), pp 590-598
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Electrical and Computer Engineering; Materials Science and Engineering; Surgery
- Web of Science ID
- WOS:000305704800006
- Scopus ID
- 2-s2.0-84862653668
- Other Identifier
- 991014878145704721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Medicine, Research & Experimental
- Nanoscience & Nanotechnology