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Piezoelectric-excited millimeter-sized cantilever biosensors
Journal article

Piezoelectric-excited millimeter-sized cantilever biosensors

Raj Mutharasan
Methods in molecular biology (Clifton, N.J.), v 504
2009
PMID: 19159091

Abstract

Immunoassay - instrumentation Reproducibility of Results Transducers Microchemistry - methods Microchemistry - instrumentation Equipment Design Protein Interaction Mapping - instrumentation Protein Interaction Mapping - methods Sensitivity and Specificity Immunoassay - methods Biosensing Techniques - instrumentation Biosensing Techniques - methods Micro-Electrical-Mechanical Systems - methods Micro-Electrical-Mechanical Systems - instrumentation Miniaturization Equipment Failure Analysis
In this chapter, method of fabricating a cantilever biosensors and their use in measuring the presence of a protein is described. There are many variations in construction of a cantilever sensor. A simple and an easy version is described in this chapter. The specificity of the sensor is obtained by immobilizing an antibody specific to the antigen of interest. The piezoelectric cantilever sensors are very sensitive and can easily detect a 60 kDa protein at 100 fg/mL concentration. Experimental procedure for carrying out detection of a target analyte is outlined and a sample set of results is included.

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