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Journal article
Plasma exosomes induced by remote ischaemic preconditioning attenuate myocardial ischaemia/reperfusion injury by transferring miR-24
Cell death & disease, v 9(3)
23 Feb 2018
PMCID: PMC5833738
PMID: 29476052
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Remote ischaemic preconditioning (RIPC) is well known to protect the myocardium against ischaemia/reperfusion injury (IRI). Exosomes are small extracellular vesicles that have become the key mediators of intercellular communication. Various studies have confirmed that circulating exosomes mediate RIPC. However, the underlying mechanisms for RIPC-induced exosome-mediated cardioprotection remain elusive. In our study, we found that the expression level of miR-24 was higher in exosomes derived from the plasma of rats subjected to RIPC than in exosomes derived from the plasma of control rats in vivo. The rat plasma exosomes could be taken up by H9c2 cells. In addition, miR-24 was present in RIPC-induced exosomes and played a role in reducing oxidative stress-mediated injury and decreasing apoptosis by downregulating Bim expression in H
O
-treated H9c2 cells in vitro. In vivo, miR-24 in RIPC-induced exosomes reduced cardiomyocyte apoptosis, attenuated the infarct size and improved heart function. Furthermore, the apoptosis-reducing effect of miR-24 was counteracted by miR-24 antagomirs or inhibitors both in vitro and in vivo. Therefore, we provided evidence that RIPC-induced exosomes could reduce apoptosis by transferring miR-24 in a paracrine manner and that miR-24 in the exosomes plays a central role in mediating the protective effects of RIPC.
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Details
- Title
- Plasma exosomes induced by remote ischaemic preconditioning attenuate myocardial ischaemia/reperfusion injury by transferring miR-24
- Creators
- Wen Minghua - Second Affiliated Hospital of Nanchang UniversityGong Zhijian - Second Affiliated Hospital of Nanchang UniversityHuang Chahua - Second Affiliated Hospital of Nanchang UniversityLiang Qiang - Jiangxi Province Key Laboratory of Molecular Medicine, Nanchang, China.Xu Minxuan - Second Affiliated Hospital of Nanchang UniversityWang Luqiao - Second Affiliated Hospital of Nanchang UniversityZhang Weifang - Second Affiliated Hospital of Nanchang UniversityLu Peng - Second Affiliated Hospital of Nanchang UniversityZhan Biming - Second Affiliated Hospital of Nanchang UniversityYu Lingling - Second Affiliated Hospital of Nanchang UniversityWang Zhenzhen - Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, ChinaXu Jianqing - Second Affiliated Hospital of Nanchang UniversityBao Huihui - Second Affiliated Hospital of Nanchang UniversityWang Xiaozhong - Second Affiliated Hospital of Nanchang UniversityCheng Xiaoshu - Second Affiliated Hospital of Nanchang University
- Publication Details
- Cell death & disease, v 9(3)
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000427414100003
- Scopus ID
- 2-s2.0-85042554769
- Other Identifier
- 991019201490404721
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