Journal article
Plasmodium Merozoite TRAP Family Protein Is Essential for Vacuole Membrane Disruption and Gamete Egress from Erythrocytes
Cell host & microbe, v 20(5), pp 618-630
09 Nov 2016
PMID: 27832590
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Surface-associated TRAP (thrombospondin-related anonymous protein) family proteins are conserved across the phylum of apicomplexan parasites. TRAP proteins are thought to play an integral role in parasite motility and cell invasion by linking the extracellular environment with the parasite submembrane actomyosin motor. Blood stage forms of the malaria parasite Plasmodium express a TRAP family protein called merozoite-TRAP (MTRAP) that has been implicated in erythrocyte invasion. Using MTRAP-deficient mutants of the rodent-infecting P. berghei and human-infecting P. falciparum parasites, we show that MTRAP is dispensable for erythrocyte invasion. Instead, MTRAP is essential for gamete egress from erythrocytes, where it is necessary for the disruption of the gamete-containing parasitophorous vacuole membrane, and thus for parasite transmission to mosquitoes. This indicates that motor-binding TRAP family members function not just in parasite motility and cell invasion but also in membrane disruption and cell egress.
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•Merozoite TRAP protein, MTRAP, is dispensable for Plasmodium asexual blood stages•MTRAP-deficient parasites are blocked from transmission to mosquitoes•MTRAP is expressed in Plasmodium sexual stages and is essential for gamete egress•MTRAP-deficient gametes fail to lyse the parasitophorous vacuole membrane for egress
MTRAP, a protein expressed in Plasmodium blood stages, was thought to function during invasion of erythrocytes by the asexual merozoite stage. Bargieri et al. report that MTRAP is dispensable for merozoite invasion but is essential for egress of the gamete sexual stage from erythrocytes and for parasite transmission to mosquitoes.
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Details
- Title
- Plasmodium Merozoite TRAP Family Protein Is Essential for Vacuole Membrane Disruption and Gamete Egress from Erythrocytes
- Creators
- Daniel Y. Bargieri - Universidade de São PauloSabine Thiberge - Institut PasteurChwen L. Tay - Imperial College LondonAlison F. Carey - Queen Saovabha Memorial InstituteAlice Rantz - Institut PasteurFlorian Hischen - RWTH Aachen UniversityAudrey Lorthiois - Délégation Paris 5Ursula Straschil - Imperial College LondonPallavi Singh - Institut PasteurShailja Singh - Institut PasteurTony Triglia - Walter and Eliza Hall Institute of Medical ResearchTakafumi Tsuboi - Ehime UniversityAlan Cowman - The University of MelbourneChetan Chitnis - Institut PasteurPietro Alano - Istituto Superiore di SanitàJake Baum - Imperial College LondonGabriele Pradel - RWTH Aachen UniversityCatherine Lavazec - Délégation Paris 5Robert Ménard - Institut PasteurAlison J Carey - College of Medicine (2002-)
- Publication Details
- Cell host & microbe, v 20(5), pp 618-630
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics; College of Medicine; Drexel University
- Web of Science ID
- WOS:000389472000011
- Scopus ID
- 2-s2.0-84994706898
- Other Identifier
- 991020100080504721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Microbiology
- Parasitology
- Virology