Journal article
Platelet-derived Growth Factor (PDGF)-induced Ca2+ Signaling in the CG4 Oligodendroglial Cell Line and in Transformed Oligodendrocytes Expressing the β-PDGF Receptor
The Journal of biological chemistry, v 272(7), pp 4351-4358
14 Feb 1997
PMID: 9020156
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Ca2+ signaling induced by platelet-derived growth factor (PDGF) was investigated in the oligodendroglial cell lines CG4 and CEINGE clone 3, using fura-2 microfluorimetry and video imaging. CEINGE cl3 cells, immortalized with polyoma middle T antigen, were found to uniformly express the polyoma middle T antigen protein as well as 2′,3′-cyclic nucleotide 3′-phosphodiesterase, a specific marker for oligodendroglia. PDGF-BB induced both oscillatory and non-oscillatory Ca2+ responses in CEINGE cl3 cells as well as in CG4 cells, grown either as O-2A progenitors or differentiated oligodendrocytes. However, in CG4 cells the percentage of oscillatory Ca2+ responses was higher than that observed in CEINGE cl3 cells. In contrast, oscillatory Ca2+ responses were not observed in PC-12 cells transfected with β-PDGF receptor (PDGFR) or in NIH 3T3 fibroblasts. CG4 cells expressed only the α-PDGFR, whereas CEINGE cl3 cells expressed both α and β isoforms. When CEINGE cl3 cells were exposed to PDGF-AA, which binds only to the α-PDGFR, the percentage of oscillatory Ca2+ responses was higher than that observed after PDGF-BB stimulation. We previously reported that block of the enzyme sphingosine kinase, and a consequent increase in intracellular sphingosine levels in CEINGE cl3 cells caused an increase in the percentage of oscillatory Ca2+ responses induced by PDGF-BB. However, in CG4 cells block of sphingosine kinase did not increase the oscillatory Ca2+ response elicited by PDGF-BB, although the addition of exogenous sphingosine induced an oscillatory Ca2+ response in 77% of cells studied. We hypothesize that the α-PDGFR is less effective than the β-PDGFR in stimulating the activity of sphingosine kinase. The results also suggest that α- and β-PDGFRs may differently regulate sphingolipid metabolism.
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Details
- Title
- Platelet-derived Growth Factor (PDGF)-induced Ca2+ Signaling in the CG4 Oligodendroglial Cell Line and in Transformed Oligodendrocytes Expressing the β-PDGF Receptor
- Creators
- Alessandro Fatatis - University of ChicagoRichard J. Miller
- Publication Details
- The Journal of biological chemistry, v 272(7), pp 4351-4358
- Publisher
- Elsevier
- Grant note
- DA-02121; MH-40165; NS-33502; DA-02575 / Public Health Service
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine; Pharmacology and Physiology; Drexel University
- Web of Science ID
- WOS:A1997WH01900072
- Scopus ID
- 2-s2.0-0031041073
- Other Identifier
- 991020099531804721
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- Web of Science research areas
- Biochemistry & Molecular Biology