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Journal article
Platelet lysate outperforms FCS and human serum for co-culture of primary human macrophages and hMSCs
Scientific reports, v 9(Mar (E-published)), pp 3533-3533
05 Mar 2019
PMID: 30837625
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In vitro co-cultures of different primary human cell types are pivotal for the testing and evaluation of biomaterials under conditions that are closer to the human in vivo situation. Especially co-cultures of macrophages and mesenchymal stem cells (MSCs) are of interest, as they are both present and involved in tissue regeneration and inflammatory reactions and play crucial roles in the immediate inflammatory reactions and the onset of regenerative processes, thus reflecting the decisive early phase of biomaterial contact with the host. A co-culture system of these cell types might thus allow for the assessment of the biocompatibility of biomaterials. The establishment of such a co-culture is challenging due to the different in vitro cell culture conditions. For human macrophages, medium is usually supplemented with human serum (hS), whereas hMSC culture is mostly performed using fetal calf serum (FCS), and these conditions are disadvantageous for the respective other cell type. We demonstrate that human platelet lysate (hPL) can replace hS in macrophage cultivation and appears to be the best option for co-cultivation of human macrophages with hMSCs. In contrast to FCS and hS, hPL maintained the phenotype of both cell types, comparable to that of their respective standard culture serum, as well as the percentage of each cell population. Moreover, the expression profile and phagocytosis activity of macrophages was similar to hS.
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Details
- Title
- Platelet lysate outperforms FCS and human serum for co-culture of primary human macrophages and hMSCs
- Creators
- Tina Tylek - Bavarian Polymer InstituteTatjana Schilling - Bavarian Polymer InstituteKatrin Schlegelmilch - Bavarian Polymer InstituteMaximilian Ries - Bavarian Polymer InstituteMaximilian Rudert - University of WürzburgFranz Jakob - University of WürzburgJuergen Groll - Univ Wurzburg, Dept Funct Mat Med & Dent, Wurzburg, Germany
- Publication Details
- Scientific reports, v 9(Mar (E-published)), pp 3533-3533
- Publisher
- Springer Nature
- Number of pages
- 11
- Grant note
- 617989 / European Research Council (ERC)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000460381600106
- Scopus ID
- 2-s2.0-85062586031
- Other Identifier
- 991019292228604721
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- Web of Science research areas
- Cell Biology