Journal article
Platelet‐Activating Factor: Diminished Acetylcholine Release from Rat Brain Slices Is Mediated by a Gi Protein
Journal of neurochemistry, v 63(5), pp 1720-1725
Nov 1994
PMID: 7931326
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
: The effect of platelet‐activating factor (PAF) on neurotransmitter release from rat brain slices prelabeled with [3H]acetylcholine ([3H]ACh), [3H]norepinephrine ([3H]NE), or [3H]serotonin ([3H]5‐HT) was studied. PAF inhibited K+ depolarization‐induced [3H]ACh release in slices of brain cortex and hippocampus by up to 59% at 10 nM but did not inhibit [3H]ACh release in striatal slices. PAF did not affect 5‐HT or NE release from cortical brain slices. The inhibition of K+‐evoked [3H]ACh release induced by PAF was prevented by pretreating tissues with several structurally different PAF receptor antagonists. The effect of PAF was reversible and was not affected by pretreating brain slices with tetrodotoxin. PAF‐induced inhibition of [3H]ACh release was blocked 90 ± 3 and 86 ± 2% by pertussis toxin and by anti‐Gαi1/2 antiserum incorporated into cortical synaptosomes, respectively. The results suggest that PAF inhibits depolarization‐induced ACh release in brain slices via a Gαi1/2 protein‐mediated action and that PAF may serve as a neuromodulator of brain cholinergic system.
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Details
- Title
- Platelet‐Activating Factor: Diminished Acetylcholine Release from Rat Brain Slices Is Mediated by a Gi Protein
- Creators
- Hoau‐Yan WangTian‐Li YueGiora Feuerstein - Drexel UniversityEitan Friedman - Drexel University
- Publication Details
- Journal of neurochemistry, v 63(5), pp 1720-1725
- Publisher
- Blackwell Science Ltd
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:A1994PN45800016
- Scopus ID
- 2-s2.0-0028036632
- Other Identifier
- 991019184100204721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences