Journal article
Polarity in Migrating Neurons Is Related to a Mechanism Analogous to Cytokinesis
Current biology, v 23(13), pp 1215-1220
08 Jul 2013
PMID: 23791725
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Migrating neurons are bipolar, with a leading process and a trailing process [1]. The proximal region of the leading process displays a concentration of F-actin that contributes to the advance of the soma and the centrosome [2–7]. Here, we show that kinesin-6, a microtubule-based motor protein best known for its role in cytokinesis, also concentrates in this region. Depletion of kinesin-6 results in multipolar neurons that either are stationary or continuously change their direction of movement. In such neurons, F-actin no longer concentrates in a single process. During cytokinesis, kinesin-6 forms a complex with a Rho-family GTPase-activating protein called MgcRacGAP to signal to the actin cytoskeleton so that cortical movements are concentrated in the cleavage furrow [8–13]. During neuronal migration, MgcRacGap also concentrates in the proximal region of the leading process, and inhibition of its activity results in a phenotype similar to kinesin-6 depletion. We conclude that neuronal migration utilizes a cytoskeletal pathway analogous to cytokinesis, with kinesin-6 signaling through MgcRacGap to the actin cytoskeleton to constrain process number and restrict protrusive activity to a single leading process, thus resulting in a bipolar neuron able to move in a directed fashion.
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•Depletion of kinesin-6 perturbs directed neuronal migration•Kinesin-6 localizes in and directs actin filaments to the proximal leading process•Depletion of kinesin-6 results in a multipolar neuron with no leading process•Mechanisms that regulate cytokinesis are repurposed for neuronal migration
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Details
- Title
- Polarity in Migrating Neurons Is Related to a Mechanism Analogous to Cytokinesis
- Creators
- Aditi Falnikar - Department of Neurobiology and Anatomy, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USAShubha Tole - Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai 400 005, IndiaMei Liu - Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, ChinaJudy S Liu - Center for Neuroscience Research, Children’s National Medical Center, 111 Michigan Avenue NW, Washington, DC 20010, USAPeter W Baas - Department of Neurobiology and Anatomy, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USA
- Publication Details
- Current biology, v 23(13), pp 1215-1220
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000321605600023
- Scopus ID
- 2-s2.0-84879906973
- Other Identifier
- 991014877650604721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biology
- Cell Biology