Journal article
Poloxamer 188 reduces axonal beading following mechanical trauma to cultured neurons
Conference proceedings (IEEE Engineering in Medicine and Biology Society. Conf.), v 2007, pp 5395-5398
2007
PMID: 18003228
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Diffuse axonal injury (DAI), a major component of traumatic brain injury, is a progressive event that may lead to secondary neuronal death. DAI is thought to be initiated by mechanically-induced increases in axolemmal permeability resulting in disruption of the cytoskeleton and blockade of axonal transport. We report an in vitro model that mimics important features of DAI observed in vivo. We induced fluid shear stress injury (FSSI) on cultured primary chick forebrain neurons and characterized the resulting structural and morphological changes. In addition, we tested the post-injury effect of Poloxamer 188 (P188), a tri-block co-polymer that is known to promote resealing membrane pores. We found that FSSI induces axonal beading, the "hallmark" morphology of DAI. Furthermore, beads co-localized with microtubule disruption, also characteristic of DAI. P188 reduced axonal beading to control levels indicating that axolemma integrity is an excellent target for therapeutic interventions.
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Details
- Title
- Poloxamer 188 reduces axonal beading following mechanical trauma to cultured neurons
- Creators
- Devrim Kilinc - Drexel University, Philadelphia, PA 19104, USA. devrim.kilinc@drexel.eduGianluca GalloKenneth Barbee
- Publication Details
- Conference proceedings (IEEE Engineering in Medicine and Biology Society. Conf.), v 2007, pp 5395-5398
- Publisher
- The Institute of Electrical and Electronics Engineers, Inc. (IEEE); United States
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000253467004084
- Scopus ID
- 2-s2.0-57649201395
- Other Identifier
- 9781424407880; 1424407885; 991014877792204721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Engineering, Biomedical
- Imaging Science & Photographic Technology
- Radiology, Nuclear Medicine & Medical Imaging