Journal article
Polyamine Effects on the NMDA Receptor in Human Brain
Experimental neurology, v 130(2), pp 323-330
01 Dec 1994
PMID: 7867761
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Polyamines are thought to modulate the activation of NMDA receptors through a unique allosteric regulatory site. The effects of polyamines on the binding of [
3H]MK-801 were measured in cortical and hippocampal tissue surgically removed from patients with temporal lobe epilepsy (TLE). The polyamine agonist spermidine increased the binding of [
3H]MK-801 in the cortex in a dose-dependent manner and this effect could be blocked by the weak partial agonist diethylenetriamine (DET). Spermidine decreased the
K
d
of [
3H]MK-801 for the NMDA receptor but did not alter the density of receptors. Spermidine had essentially the same effect on
K
d
and
B
max measured in the dentate gyrus of TLE subjects and the cortex and dentate gyrus of postmortem controls. Moreover, there was no difference in the density of binding sites between postmortem and TLE subjects in either region. The binding of [
3H]MK-801 in human cortex was decreased by 30% by incubation with DET or by prewashing the tissue sections. In contrast, DET did not alter the binding of [
3H]MK-801 in rat cortex and prewashing sections produced an increase rather than a decrease in binding. These results suggest that there are different endogenous modulators for the polyamine site in rat and human tissue. The inverse agonist 1,10-diaminodecane decreased the binding of [
3H]MK-801 in a dose-dependent manner. These results suggest that the fundamental modulatory properties of polyamines in rat and human tissues are essentially the same and that endogenous polyamines may regulate human NMDA receptors.
Metrics
Details
- Title
- Polyamine Effects on the NMDA Receptor in Human Brain
- Creators
- Swaminathan Subramaniam - University of PennsylvaniaMichael J. O'ConnorLeona M. MasukawaPaul McGonigle
- Publication Details
- Experimental neurology, v 130(2), pp 323-330
- Publisher
- Elsevier
- Number of pages
- 8
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:A1994QJ16200015
- Scopus ID
- 2-s2.0-0028646234
- Other Identifier
- 991021902507304721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Neurosciences