Journal article
Polynucleotide Adsorption to Negatively Charged Surfaces in Divalent Salt Solutions
Biophysical journal, v 90(4), pp 1164-1174
15 Feb 2006
PMID: 16449197
Abstract
Polynucleotide adsorption to negatively charged surfaces via divalent ions is extensively used in the study of biological systems. We analyze here the adsorption mechanism via a self-consistent mean-field model that includes the pH effect on the surface-charge density and the interactions between divalent ions and surface groups. The adsorption is driven by the cooperative effect of divalent metal ion condensation along polynucleotides and their reaction with the surface groups. Although the apparent reaction constants are enhanced by the presence of polynucleotides, the difference between reaction constants of different divalent ions at the ideal condition explains why not all divalent cations mediate DNA adsorption onto anionic surfaces. Calculated divalent salt concentration and pH value variations on polynucleotide adsorption are consistent with atomic force microscope results. Here we use long-period x-ray standing waves to study the adsorption of mercurated-polyuridylic acid in a ZnCl
2
aqueous solution onto a negatively charged hydroxyl-terminated silica surface. These in situ x-ray measurements, which simultaneously reveal the Hg and Zn distribution profiles along the surface normal direction, are in good agreement with our model. The model also provides the effects of polyelectrolyte line-charge density and monovalent salt on adsorption.
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Details
- Title
- Polynucleotide Adsorption to Negatively Charged Surfaces in Divalent Salt Solutions
- Creators
- Hao Cheng - Northwestern UniversityKai Zhang - Northwestern UniversityJoseph A. Libera - Northwestern UniversityMonica Olvera de la Cruz - Department of Materials Science and Engineering, Northwestern University, Evanston, IllinoisMichael J. Bedzyk - Northwestern University
- Publication Details
- Biophysical journal, v 90(4), pp 1164-1174
- Publisher
- Biophysical Society
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Materials Science and Engineering
- Web of Science ID
- WOS:000235004200009
- Scopus ID
- 2-s2.0-33645791711
- Other Identifier
- 991021886794204721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biophysics