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Pooling Bio-Specimens in the Presence of Measurement Error and Non-Linearity in Dose-Response: Simulation Study in the Context of a Birth Cohort Investigating Risk Factors for Autism Spectrum Disorders
Journal article   Open access   Peer reviewed

Pooling Bio-Specimens in the Presence of Measurement Error and Non-Linearity in Dose-Response: Simulation Study in the Context of a Birth Cohort Investigating Risk Factors for Autism Spectrum Disorders

Karyn Heavner, Craig Newschaffer, Irva Hertz-Picciotto, Deborah Bennett and Igor Burstyn
International journal of environmental research and public health, v 12(11), pp 14780-14799
19 Nov 2015
PMID: 26610532
url
https://doi.org/10.3390/ijerph121114780View
Published, Version of Record (VoR) Open

Abstract

Meta-Analysis as Topic Bias Humans Risk Factors Linear Models Logistic Models Male Environmental Exposure Models, Statistical False Positive Reactions Sample Size Computer Simulation Female Autism Spectrum Disorder - etiology Research Design Cohort Studies
We sought to determine the potential effects of pooling on power, false positive rate (FPR), and bias of the estimated associations between hypothetical environmental exposures and dichotomous autism spectrum disorders (ASD) status. Simulated birth cohorts in which ASD outcome was assumed to have been ascertained with uncertainty were created. We investigated the impact on the power of the analysis (using logistic regression) to detect true associations with exposure (X₁) and the FPR for a non-causal correlate of exposure (X₂, r = 0.7) for a dichotomized ASD measure when the pool size, sample size, degree of measurement error variance in exposure, strength of the true association, and shape of the exposure-response curve varied. We found that there was minimal change (bias) in the measures of association for the main effect (X₁). There is some loss of power but there is less chance of detecting a false positive result for pooled compared to individual level models. The number of pools had more effect on the power and FPR than the overall sample size. This study supports the use of pooling to reduce laboratory costs while maintaining statistical efficiency in scenarios similar to the simulated prospective risk-enriched ASD cohort.

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Collaboration types
Domestic collaboration
Web of Science research areas
Environmental Sciences
Public, Environmental & Occupational Health
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