Journal article
Porcine intact and wounded skin responses to atmospheric nonthermal plasma
The Journal of surgical research, v 179(1), pp e1-e12
Jan 2013
PMID: 22480830
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Thermal plasma is a valued tool in surgery for its coagulative and ablative properties. We suggested through in vitro studies that nonthermal plasma can sterilize tissues, inactive pathogens, promote coagulation, and potentiate wound healing. The present research was undertaken to study acute toxicity in porcine skin tissues. We demonstrate that floating electrode-discharge barrier discharge (FE-DBD) nonthermal plasma is electrically safe to apply to living organisms for short periods. We investigated the effects of FE-DBD plasma on Yorkshire pigs on intact and wounded skin immediately after treatment or 24h posttreatment. Macroscopic or microscopic histological changes were identified using histological and immunohistochemical techniques. The changes were classified into four groups for intact skin: normal features, minimal changes or congestive changes, epidermal layer damage, and full burn and into three groups for wounded skin: normal, clot or scab, and full burn-like features. Immunohistochemical staining for laminin layer integrity showed compromise over time. A marker for double-stranded DNA breaks, γ-H2AX, increased over plasma-exposure time. These findings identified a threshold for plasma exposure of up to 900s at low power and <120s at high power. Nonthermal FE-DBD plasma can be considered safe for future studies of external use under these threshold conditions for evaluation of sterilization, coagulation, and wound healing.
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Details
- Title
- Porcine intact and wounded skin responses to atmospheric nonthermal plasma
- Creators
- Andrew S Wu - Department of Surgery, Drexel University College of Medicine, 245 N 15th Street, Mail Stop 413, NCB Suite 7150, Philadelphia, Pennsylvania 19102Sameer Kalghatgi - A. J. Drexel Plasma Institute, Drexel University, Philadelphia, Pennsylvania 19104Danil Dobrynin - A. J. Drexel Plasma Institute, Drexel University, Philadelphia, Pennsylvania 19104Rachel Sensenig - Department of Surgery, Drexel University College of Medicine, 245 N 15th Street, Mail Stop 413, NCB Suite 7150, Philadelphia, Pennsylvania 19102Ekaternia Cerchar - Department of Surgery, Drexel University College of Medicine, 245 N 15th Street, Mail Stop 413, NCB Suite 7150, Philadelphia, Pennsylvania 19102Erica Podolsky - Department of Surgery, Drexel University College of Medicine, 245 N 15th Street, Mail Stop 413, NCB Suite 7150, Philadelphia, Pennsylvania 19102Essel Dulaimi - Department of Pathology & Laboratory Medicine, Drexel University College of Medicine; 245 N 15th Street, Mail Stop 413, NCB Suite 7150, Philadelphia, Pennsylvania 19102Michelle Paff - Department of Surgery, Drexel University College of Medicine, 245 N 15th Street, Mail Stop 413, NCB Suite 7150, Philadelphia, Pennsylvania 19102Kimberly Wasko - Department of Surgery, Drexel University College of Medicine, 245 N 15th Street, Mail Stop 413, NCB Suite 7150, Philadelphia, Pennsylvania 19102Krishna Priya Arjunan - A. J. Drexel Plasma Institute, Drexel University, Philadelphia, Pennsylvania 19104Kristin Garcia - Department of Surgery, Drexel University College of Medicine, 245 N 15th Street, Mail Stop 413, NCB Suite 7150, Philadelphia, Pennsylvania 19102Gregory Fridman - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, Pennsylvania 19104Manjula Balasubramanian - Department of Pathology & Laboratory Medicine, Drexel University College of Medicine; 245 N 15th Street, Mail Stop 413, NCB Suite 7150, Philadelphia, Pennsylvania 19102Robert Ownbey - Department of Pathology & Laboratory Medicine, Drexel University College of Medicine; 245 N 15th Street, Mail Stop 413, NCB Suite 7150, Philadelphia, Pennsylvania 19102Kenneth A Barbee - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, Pennsylvania 19104Alexander Fridman - A. J. Drexel Plasma Institute, Drexel University, Philadelphia, Pennsylvania 19104Gary Friedman - A. J. Drexel Plasma Institute, Drexel University, Philadelphia, Pennsylvania 19104Suresh G Joshi - Department of Surgery, Drexel University College of Medicine, 245 N 15th Street, Mail Stop 413, NCB Suite 7150, Philadelphia, Pennsylvania 19102Ari D Brooks - Department of Surgery, Drexel University College of Medicine, 245 N 15th Street, Mail Stop 413, NCB Suite 7150, Philadelphia, Pennsylvania 19102
- Publication Details
- The Journal of surgical research, v 179(1), pp e1-e12
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Electrical and Computer Engineering; School of Biomedical Engineering, Science, and Health Systems; Mechanical Engineering and Mechanics; Surgery
- Web of Science ID
- WOS:000312129700001
- Scopus ID
- 2-s2.0-84870674764
- Other Identifier
- 991014877676404721
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- Web of Science research areas
- Surgery