Journal article
Positive and negative hepatitis C virus RNA strands in serum, liver and peripheral blood mononuclear cells in anti-HCV patients: relation with the liver lesion
Journal of hepatology, v 21(2), pp 182-186
01 Aug 1994
PMID: 7989707
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
To investigate the replicative hepatitis C virus status and its relation to liver damage, serum, peripheral blood mononuclear cells and liver-paired samples from 45 untreated hepatitis C virus infected patients (38 with chronic hepatitis, three with minimal changes, and four with normal liver) were studied by nested polymerase chain reaction, using primers from the 5′ untranslated region. Positive HCV-RNA strand was detected in serum (69%), peripheral blood mononuclear cells (100%) and liver samples (100%). The presence of negative HCV-RNA strand was confirmed using specificity controls assays and was only detected in liver and peripheral blood mononuclear cells samples, (95% and 82%, respectively). No correlation between the presence of negative HCV-RNA strand in peripheral blood mononuclear cells and positive HCV-RNA strand in serum was found, whereas serum HCV-RNA was not detected in patients without negative HCV-RNA strand in the liver. Both positive and negative HCV-RNA strands were found in liver and peripheral blood mononuclear cells of four patients with normal liver histology, and three with minimal changes. Furthermore, the presence of HCV-RNA in serum did not correlate with the alanine aminotransferase values and the histological activity index. These data confirm the existence of replicative intermediates in the liver, not only from patients with histologically proven chronic hepatitis, but also from those with normal liver, suggesting the existence of hepatitis C virus in true healthy carriers.
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Details
- Title
- Positive and negative hepatitis C virus RNA strands in serum, liver and peripheral blood mononuclear cells in anti-HCV patients: relation with the liver lesion
- Creators
- Inmaculada Castillo - Hospital Universitario Fundación Jiménez DíazJavier Bartolomé - Hospital Universitario Fundación Jiménez DíazEduardo Marriott - Hospital Universitario Fundación Jiménez DíazMonserrat Herrero - Hospital Universitario Fundación Jiménez DíazSonia Navas-Martin - Drexel University, Microbiology and ImmunologyVicente Carreño - Fundación para el Estudio de las Hepatitis Virales
- Publication Details
- Journal of hepatology, v 21(2), pp 182-186
- Publisher
- Elsevier
- Number of pages
- 5
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1994PD99100007
- Scopus ID
- 2-s2.0-0027959406
- Other Identifier
- 991022047818304721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Gastroenterology & Hepatology