Journal article
Positron emission tomography probe demonstrates a striking concentration of ribose salvage in the liver
Proceedings of the National Academy of Sciences - PNAS, v 111(28), pp E2866-E2874
15 Jul 2014
PMID: 24982199
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
PET is a powerful technique for quantifying and visualizing biochemical pathways in vivo. Here, we develop and validate a novel PET probe, [(18)F]-2-deoxy-2-fluoroarabinose ([(18)F]DFA), for in vivo imaging of ribose salvage. DFA mimics ribose in vivo and accumulates in cells following phosphorylation by ribokinase and further metabolism by transketolase. We use [(18)F]DFA to show that ribose preferentially accumulates in the liver, suggesting a striking tissue specificity for ribose metabolism. We demonstrate that solute carrier family 2, member 2 (also known as GLUT2), a glucose transporter expressed in the liver, is one ribose transporter, but we do not know if others exist. [(18)F]DFA accumulation is attenuated in several mouse models of metabolic syndrome, suggesting an association between ribose salvage and glucose and lipid metabolism. These results describe a tool for studying ribose salvage and suggest that plasma ribose is preferentially metabolized in the liver.
Metrics
Details
- Title
- Positron emission tomography probe demonstrates a striking concentration of ribose salvage in the liver
- Creators
- Peter M Clark - Czech Academy of Sciences, Institute of Molecular GeneticsGraciela Flores - Molecular and Medical Pharmacology,Crump Institute for Molecular Imaging.Nikolai M Evdokimov - Molecular and Medical Pharmacology,Chemistry and Biochemistry, and.Melissa N McCracken - Molecular and Medical Pharmacology.Timothy Chai - Departments of Microbiology, Immunology, and Molecular Genetics.Evan Nair-Gill - Molecular and Medical Pharmacology.Fiona O'Mahony - Division of Digestive DiseasesSimon W Beaven - Division of Digestive Diseases.Kym F Faull - Semel Institute for Neuroscience and Human BehaviorMichael E Phelps - bMolecular and Medical Pharmacology,Michael E Jung - Chemistry and Biochemistry, and.Owen N Witte - Czech Academy of Sciences, Institute of Molecular Genetics
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, v 111(28), pp E2866-E2874
- Publisher
- PNAS
- Grant note
- P30 CA016042 / NCI NIH HHS Howard Hughes Medical Institute R25 CA098010 / NCI NIH HHS P50 CA086306 / NCI NIH HHS R25T CA098010 / NCI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Drexel University
- Web of Science ID
- WOS:000338985700009
- Scopus ID
- 2-s2.0-84904303858
- Other Identifier
- 991019356496404721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology