Journal article
Possible orientational constraints determine secretory signals induced by aggregation of IgE receptors on mast cells
The EMBO journal, v 7(13), pp 4101-4109
20 Dec 1988
PMID: 2977332
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Three biologically active monoclonal antibodies (mAbs) specific for the monovalent, high-affinity membrane receptor for IgE (Fc epsilon R) were employed in analysing the secretory response of mast cells of the RBL-2H3 line to crosslinking of their Fc epsilon R. All three mAbs (designated F4, H10 and J17) compete with each other and with IgE for binding to the Fc epsilon R. Their stoichiometry of binding is 1 Fab:1 Fc epsilon R, hence, the intact mAbs can aggregate the Fc epsilon Rs to dimers only. Since all three mAbs induce secretion, we conclude that Fc epsilon R dimers constitute a sufficient 'signal element' for secretion of mediators for RBL-2H3 cells. The secretory dose-response of the cells to these three mAbs are, however, markedly different: F4 caused rather high secretion, reaching almost 80% of the cells' content, while J17 and H10 induced release of only 30-40% mediators content. Both the intrinsic affinities and equilibrium constants for the receptor dimerization were derived from analysis of binding data of the Fab fragments and intact mAbs. These parameters were used to compute the extent of Fc epsilon R dimerization caused by each of the antibodies. However, the different secretory responses to the three mAbs could not be rationalized simply in terms of the extent of Fc epsilon R dimerization which they produce. This suggests that it is not only the number of crosslinked Fc epsilon Rs which determines the magnitude of secretion-causing signal, but rather other constraints imposed by each individual mAb are also important.
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Details
- Title
- Possible orientational constraints determine secretory signals induced by aggregation of IgE receptors on mast cells
- Creators
- E Ortega - Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, IsraelR Schweitzer-Stenner - Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, IsraelI Pecht - Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel
- Publication Details
- The EMBO journal, v 7(13), pp 4101-4109
- Publisher
- Wiley
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:A1988R465900010
- Scopus ID
- 2-s2.0-0024293527
- Other Identifier
- 991014878118404721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology