Journal article
Postsynaptic target regulates functional responses induced by 5-HT 3 serotonin receptors on axonal varicosities of NG108-15 hybrid neuroblastoma cells
Neuroscience, v 102(4), pp 979-987
2001
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Rat brain presynaptic 5-HT
3 serotonin receptors, members of the ligand-gated ion channel superfamily, induce changes in nerve terminal [Ca
2+]
i in a manner distinct from that found for somatic 5-HT
3 receptors. Here, we assessed the role of postsynaptic target in regulating the nature of presynaptic receptor-induced responses, using the hybrid neuroblastoma cell line NG108-15 as a model neuronal system that expresses 5-HT
3 receptors. Using immunocytochemistry, 5-HT
3 receptors were found to be present on the presynaptic-like varicosities of differentiated NG108-15 cells, indicating that these receptors possess an inherent ability to localize to potential presynaptic sites. In the absence of postsynaptic target, 5-HT
3 receptors localized to the varicosities induce rapid but transient changes in [Ca
2+]
i that were initiated by voltage-gated Ca
2+ channels, as assessed using Ca
2+ channel blockers, these properties being typical of those found for somatic 5-HT
3 receptors. In co-cultures containing rat myotubes, with which NG108-15 cells form functional cholinergic synapses, the 5-HT
3 receptor-induced changes in [Ca
2+]
i in the axonal varicosities shifted over time (three to 10 days) to that found for brain nerve endings: sustained responses that were insensitive to blockade by antagonists of voltage-gated Ca
2+ channels. The effect of co-culturing myotubes with the NG108-15 cells was mimicked by conditioned media from myotube cultures.
These results indicate that regulatory molecules from the target postsynaptic cell dictate the functional responses elicited by presynaptic 5-HT
3 receptors. Because the target-induced changes required several days before they were evident, we hypothesize that changes in protein expression, perhaps the consequence of altered gene regulation, underlie the changes in the responses to 5-HT
3 receptor activation in the axonal varicosities of this neuronal cell line.
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Details
- Title
- Postsynaptic target regulates functional responses induced by 5-HT 3 serotonin receptors on axonal varicosities of NG108-15 hybrid neuroblastoma cells
- Creators
- P. Rondé - Drexel UniversityR.A. Nichols - Drexel University
- Publication Details
- Neuroscience, v 102(4), pp 979-987
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000167232300021
- Scopus ID
- 2-s2.0-0035857437
- Other Identifier
- 991019350667404721
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- Web of Science research areas
- Neurosciences