Journal article
Potential Roles of Antiphospholipid Antibodies in Generating Platelet-C4d in Systemic Lupus Erythematosus
Antibodies (Basel), v 6(3), p9
02 Jul 2017
PMID: 31548524
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Premature, accelerated onset of atherothrombotic disease is prevalent in patients with systemic lupus erythematosus (SLE). Most, if not all, atherothrombotic diseases are likely to involve platelets and complement. Previously, we discovered that platelets bearing complement activation product C4d (P-C4d) are present in SLE patients, and are significantly associated with antiphospholipid (aPL) antibody positivity and stroke in SLE patients. The goal of the present study was to further elucidate the role of aPL and other platelet-reactive autoantibodies in the generation of P-C4d. To determine the association between P-C4d and aPL antibodies, the serum levels of aPL antibodies and P-C4d of 180 SLE patients were measured by enzyme-linked immunoassays and flow cytometry, respectively. To investigate the role of aPL antibodies, and possibly other autoantibodies as well, in mediating the generation of P-C4d, in vitro 2-step P-C4d induction experiments were performed. The results showed that the presence and levels of aPL antibodies in the serum were specifically elevated in SLE patients with positive P-C4d. The plasma and immunoglobulins purified from SLE patients who were positive for P-C4d and aPL were capable of inducing C4d deposition on normal platelets in vitro. The capacity of SLE plasma in inducing P-C4d appeared to correlate proportionately to the serum aPL levels. Collectively, the results demonstrate that both aPL and other platelet-reactive autoantibodies may participate in mediating the generation of P-C4d in SLE patients.
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Details
- Title
- Potential Roles of Antiphospholipid Antibodies in Generating Platelet-C4d in Systemic Lupus Erythematosus
- Creators
- Chau-Ching Liu - Allegheny Hlth Network, Allegheny Hlth Network Res Inst, Autoimmun Inst, Lupus Ctr Excellence, Pittsburgh, PA 15212 USATravis Schofield - Allegheny Health NetworkAmy Tang - Allegheny Health NetworkSusan Manzi - Allegheny Health NetworkJoseph M. Ahearn - Allegheny Health Network
- Publication Details
- Antibodies (Basel), v 6(3), p9
- Publisher
- MDPI AG
- Number of pages
- 16
- Grant note
- W81XWH-06-2-0038 / Department of Defense; United States Department of Defense
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Medicine (Graduate); General Internal Medicine
- Web of Science ID
- WOS:000416764900001
- Scopus ID
- 2-s2.0-85106270851
- Other Identifier
- 991021934003004721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Immunology