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Potential switch from eupnea to fictive gasping after blockade of glycine transmission and potassium channels
Journal article   Peer reviewed

Potential switch from eupnea to fictive gasping after blockade of glycine transmission and potassium channels

Walter M St.-John, Ilya A Rybak and Julian F. R Paton
American journal of physiology. Regulatory, integrative and comparative physiology, v 283(3), pp R721-R731
01 Sep 2002
PMID: 12185007

Abstract

This study evaluated possible neuronal mechanisms responsible for the transition from normal breathing (eupnea) to gasping. We hypothesized that a blockade of both inhibitory glycinergic synaptic transmission and potassium channels, combined with an increase in extracellular concentration of potassium, would induce a switch from an eupneic respiratory pattern to gasping. Efferent activities of the phrenic, vagal, and hypoglossal nerves were recorded during eupnea and ischemia-induced gasping in a perfused in situ preparation of the juvenile rat (4–6 wk of age). To block potassium channels, 4-aminopyridine (4-AP, 1–10 μM) was administered. Strychnine (0.2–0.6 μM) was used to block glycinergic neurotransmission. After administrations of 4-AP, excess extracellular potassium (10.25–17.25 mM), and strychnine, the incrementing pattern of eupneic phrenic activity was altered to a decrementing discharge. Hypoglossal and vagal activities became concentrated to the period of the phrenic burst with expiratory activity being reduced or eliminated. These changes in neural activities were similar to those in ischemia-induced gasping. Results are consistent with the concept that the elicitation of gasping represents a switch from a network-based rhythmogenesis for eupnea to a pacemaker-driven mechanism.

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Physiology
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