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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Practical NK cell phenotyping and variability in healthy adults
Immunologic research, v 62(3), pp 341-356
2015
PMID: 26013798
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Human natural killer (NK) cells display a wide array of surface and intracellular markers that indicate various states of differentiation and/or levels of effector function. These NK cell subsets exist simultaneously in peripheral blood and may vary among individuals. We examined variety among selected NK cell receptors expressed by NK cells from normal donors, as well as the distribution of select NK cell subsets and NK cell receptor expression over time in several individual donors. Peripheral blood mononuclear cells were evaluated using flow cytometry via fluorochrome-conjugated antibodies against a number of NK cell receptors. Results were analyzed for both mean fluorescence intensity (MFI) and the percent positive cells for each receptor. CD56
bright
and CD56
dim
NK cell subsets were also considered separately, as was variation in receptor expression in NK cell subsets over time in selected individuals. Through this effort, we provide ranges of NK cell surface receptor expression for a local adult population as well as provide insight into intra-individual variation.
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Details
- Title
- Practical NK cell phenotyping and variability in healthy adults
- Creators
- Laura S. Angelo - Baylor College of MedicinePinaki P. Banerjee - Baylor College of MedicineLinda Monaco-Shawver - Children's Hospital of PhiladelphiaJoshua B. Rosen - Drexel UniversityGeorge Makedonas - Baylor College of MedicineLisa R. Forbes - Baylor College of MedicineEmily M. Mace - Baylor College of MedicineJordan S. Orange - Baylor College of MedicineJanell L Mensinger
- Publication Details
- Immunologic research, v 62(3), pp 341-356
- Publisher
- Springer US
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000356270100009
- Scopus ID
- 2-s2.0-84931576769
- Other Identifier
- 991019357635704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology