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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Precise surface functionalization of PLGA particles for human T cell modulation
Nature Protocols, v 18(11), pp 3289-3321
01 Nov 2023
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The biofunctionalization of synthetic materials has extensive utility for biomedical applications, but approaches to bioconjugation typically show insufficient efficiency and controllability. We recently developed an approach by building synthetic DNA scaffolds on biomaterial surfaces that enables the precise control of cargo density and ratio, thus improving the assembly and organization of functional cargos. We used this approach to show that the modulation and phenotypic adaptation of immune cells can be regulated using our precisely functionalized biomaterials. Here, we describe the three key procedures, including the fabrication of polymeric particles engrafted with short DNA scaffolds, the attachment of functional cargos with complementary DNA strands, and the surface assembly control and quantification. We also explain the critical checkpoints needed to ensure the overall quality and expected characteristics of the biological product. We provide additional experimental design considerations for modifying the approach by varying the material composition, size or cargo types. As an example, we cover the use of the protocol for human primary T cell activation and for the identification of parameters that affect ex vivo T cell manufacturing. The protocol requires users with diverse expertise ranging from synthetic materials to bioconjugation chemistry to immunology. The fabrication procedures and validation assays to design high-fidelity DNA-scaffolded biomaterials typically require 8 d.
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7 citations in Web of Science
6 citations in Scopus
Details
- Title
- Precise surface functionalization of PLGA particles for human T cell modulation
- Creators
- Pierce HadleyYuanzhou ChenLariana ClineZhiyuan HanQizhi TangXiao Huang - Drexel University, School of Biomedical Engineering, Science, and Health SystemsTejal Desai
- Publication Details
- Nature Protocols, v 18(11), pp 3289-3321
- Publisher
- eScholarship, University of California
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Other Identifier
- 991021861308804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods