Journal article
Precision extruding deposition (PED) fabrication of polycaprolactone (PCL) scaffolds for bone tissue engineering
Biofabrication, v 1(1), pp 015003-015003
01 Mar 2009
PMID: 20811098
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Bone tissue engineering is an emerging field providing viable substitutes for bone regeneration. Recent advances have allowed scientists and engineers to develop scaffolds for guided bone growth. However, success requires scaffolds to have specific macroscopic geometries and internal architectures conducive to biological and biophysical functions. Freeform fabrication provides an effective process tool to manufacture three-dimensional porous scaffolds with complex shapes and designed properties. A novel precision extruding deposition (PED) technique was developed to fabricate polycaprolactone (PCL) scaffolds. It was possible to manufacture scaffolds with a controlled pore size of 350 mu m with designed structural orientations using this method. The scaffold morphology, internal micro-architecture and mechanical properties were evaluated using scanning electron microscopy (SEM), micro-computed tomography (micro-CT) and mechanical testing, respectively. An in vitro cell-scaffold interaction study was carried out using primary fetal bovine osteoblasts. Specifically, the cell proliferation and differentiation was evaluated by Alamar Blue assay for cell metabolic activity, alkaline phosphatase activity and osteoblast production of calcium. An in vivo study was performed on nude mice to determine the capability of osteoblast-seeded PCL to induce osteogenesis. Each scaffold was implanted subcutaneously in nude mice and, following sacrifice, was explanted at one of a series of time intervals. The explants were then evaluated histologically for possible areas of osseointegration. Microscopy and radiological examination showed multiple areas of osseous ingrowth suggesting that the osteoblast-seeded PCL scaffolds evoke osteogenesis in vivo. These studies demonstrated the viability of the PED process to fabricate PCL scaffolds having the necessary mechanical properties, structural integrity, and controlled pore size and interconnectivity desired for bone tissue engineering.
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Details
- Title
- Precision extruding deposition (PED) fabrication of polycaprolactone (PCL) scaffolds for bone tissue engineering
- Creators
- Lauren Shor - Drexel UniversitySelcuk Gueceri - Drexel Univ, Dept Mech Engn & Mech, Philadelphia, PA 19104 USARobert Chang - Drexel UniversityJennifer Gordon - Medical University of South CarolinaQian Kang - Medical University of South CarolinaLangdon Hartsock - Medical University of South CarolinaYuehuei An - Medical University of South CarolinaWei Sun - Drexel University
- Publication Details
- Biofabrication, v 1(1), pp 015003-015003
- Publisher
- Iop Publishing Ltd
- Number of pages
- 10
- Grant note
- NSF ITR 0427216 / NSF; National Science Foundation (NSF)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000278082100004
- Scopus ID
- 2-s2.0-77956761652
- Other Identifier
- 991019167653504721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials