Journal article
Predicting the CD34 content of mobilized peripheral blood leukapheresis products: single institution experience over 20 years
CYTOTHERAPY, v 26(2), p171
Feb 2024
PMID: 37930293
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background aims: Since the standardization of CD34 measurement by flow cytometry, predictors of leukapheresis CD34 yield have played a pivotal role in planning donor leukaphereses. We describe here a single institution's experience with a multivariate predictor that was used for 2,929 products without alteration for 20 years. Methods: The ordinary least squares regression model variables included log peripheral CD34 count, collection duration (3- versus 4 -hours), collection number, donor sex, and transplant type. Results: During the study period we changed flow cytometers twice and leukapheresis instruments once. During the Cobe Spectra era the predictor explained 90% of the variability in CD34 collection yield for autologous transplants (r(2) = 0.90), and 70% for allogeneic transplants with an overall sensitivity to predict a CD34 yield of >= 1 x 10(6)/kg of 97.7%, and specificity of 81.4%. Conclusions: Implemented prospectively with real-time result reporting, the model allowed us to predict CD34 yield with both 3- and 4 -hour collection scenarios. Given this guidance, 3 -hour collections were selected by the clinical team 25% of the time, saving patient leukapheresis time and resources. When faced with a prediction of <1 x 10(6) CD34/kg, the clinical team chose to defer collection 72% of the time. In instances where leukapheresis was performed despite a poor predicted outcome, 85% of patients collected on the Cobe Spectra, and 92% of patients collected on the Optia, failed to collect at least 1 x 10(6) CD34/kg. A revised model is tested retrospectively on Optia data, and suggestions for further improvements are discussed. (c) 2023 International Society for Cell & Gene Therapy. Published by Elsevier Inc. All rights reserved.
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Details
- Title
- Predicting the CD34 content of mobilized peripheral blood leukapheresis products: single institution experience over 20 years
- Publication Details
- CYTOTHERAPY, v 26(2), p171
- Publisher
- ELSEVIER SCI LTD; London
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Drexel University
- Web of Science ID
- WOS:001170787300001
- Scopus ID
- 2-s2.0-85176240466
- Other Identifier
- 991021861164804721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Cell & Tissue Engineering
- Cell Biology
- Hematology
- Medicine, Research & Experimental