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Journal article
Prediction of Human Immunodeficiency Virus Type 1 Subtype-Specific Off-Target Effects Arising from CRISPR-Cas9 Gene Editing Therapy
CRISPR journal, v 1(4)
01 Aug 2018
PMID: 31021222
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Chronic human immunodeficiency virus type 1 (HIV-1) disease is characterized by the retention of provirus within latently infected cells. Anti-HIV-1 CRISPR-Cas9 gene editing is an attractive strategy to excise or inactivate the HIV-1 genome. Recent strategies have focused on designing gRNAs that target the long terminal repeat (LTR) because 5′ and 3′ LTR symmetry can facilitate proviral excision. However, the promiscuity of CRISPR-Cas9 gene editing system necessitates the investigation of potential off-target effects. Here, potential gRNAs designed from HIV-1 phylogenetic subtypes using the CRISPRseek tool were investigated. Across the LTR, it was found that certain regions show higher human homology than others. When using recommended cutoffs, 96.40% of gRNAs were predicted to have no high probability off-target effects. Given this observation, while high-probability off-target effects are a potential danger, they can be avoided with proper gRNA design.
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Details
- Title
- Prediction of Human Immunodeficiency Virus Type 1 Subtype-Specific Off-Target Effects Arising from CRISPR-Cas9 Gene Editing Therapy
- Creators
- Robert W. Link - Drexel UniversityMichael R. Nonnemacher - Drexel UniversityBrian Wigdahl - Drexel UniversityWill Dampier - Drexel University
- Publication Details
- CRISPR journal, v 1(4)
- Publisher
- Mary Ann Liebert, Inc
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000457716600013
- Other Identifier
- 991019169910704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Genetics & Heredity