Journal article
Preproenkephalin A Gene Expression in Rat Pineal
Neuroendocrinology, v 54(6), pp 594-598
1991
PMID: 1723788
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Preproenkephalin A (ppEnk) mRNA has been identified in extracts of rat pineal gland using oligonucleotides complementary to nonoverlapping regions of this mRNA. The mRNA has an apparent molecular size of 1.4 kb, equivalent to that found in rat brain. Slot-blot analysis, used to determine the relative amount of ppEnk mRNA in serveral tissues, demonstrated that the pineal contains 70% of the concentration of ppEnk mRNA found in the hypothalamus. Using a highly specific radioimmunoassay, rat pineals were demonstrated to contain approximately 3.5 pg methionine-enkephalin (Met-Enk) per gland. Following enzymatic digestion to release cryptic Met-Enk from larger molecular forms, pineals contained approximately 160 pg Met-Enk per gland. Gel filtration analysis of pineal extracts demonstrated that the peak of the cryptic Met-Enk has a molecular weight of 32 kDa (presumably proenkephalin). These results suggest that in rat pineal ppEnk gene products may contribute to pineal functioning.
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Details
- Title
- Preproenkephalin A Gene Expression in Rat Pineal
- Creators
- Vincent J Aloyo - Drexel University
- Publication Details
- Neuroendocrinology, v 54(6), pp 594-598
- Number of pages
- 5
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:A1991GT45100009
- Scopus ID
- 2-s2.0-0025944849
- Other Identifier
- 991019184082104721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Endocrinology & Metabolism
- Neurosciences