Logo image
Back
Presence of bacteria in failed anterior cruciate ligament reconstructions
Journal article   Open access   Peer reviewed

Presence of bacteria in failed anterior cruciate ligament reconstructions

N. Luisa Hiller, Aakash Chauhan, Michael Palmer, Sameer Jain, Nicholas G. Sotereanos, Gregory T. Altman, Laura Nistico, Rachael Kreft, J. Christopher Post, Patrick J. Demeo, …
SpringerPlus, v 4(1), pp 460-460
28 Aug 2015
DOI: https://doi.org/10.1186/s40064-015-1213-2
PMID: 26339561
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1186/s40064-015-1213-2View
Published, Version of Record (VoR)CC BY V4.0,  Open

Abstract

Multidisciplinary Sciences Science & Technology Science & Technology - Other Topics
Background: Novel microbial detection technologies have revealed that chronic bacterial biofilms, which are recalcitrant to antibiotic treatment, are common in failed orthopedic procedures. Questions: Are bacteria present on failed anterior cruciate ligament (ACL) reconstructions? Is there a difference in the presence or nature of bacteria in failed ACL reconstructions relative to a control set of healthy ACL's? Methods: We used a case-control study design, where we analyzed the bacterial composition of 10 failed ACL reconstructions and compared it to 10 native ACL's harvested during total knee arthroplasty. The IBIS Universal Biosensor was used to determine the nature of bacteria on ACL specimens, and fluorescent in situ hybridization (FISH) was used to visualize bacteria in a subset of cases. Results: Bacteria are present in failed ACL reconstructions. Bacteria are present in ACL's harvested during total knee arthroplasty, but the nature of the species differs significantly between experimental and control sets. Twelve genera were detected in the experimental set (in both allografts and autografts), and in four samples multiple species were detected. In contrast, the control group was characterized by presence of Propionibacterium acnes. Conclusions: We demonstrate the presence of bacteria on failed ACLs surgeries, and open the door to investigate whether and how bacteria and the associated immune responses could possibly contribute to graft failure.

Metrics

24 Record Views
16 citations in Web of Science
17 citations in Scopus

Details

UN Sustainable Development Goals (SDGs)

This publication has contributed to the advancement of the following goals:

#5 Gender Equality

InCites Highlights

Data related to this publication, from InCites Benchmarking & Analytics tool:

Collaboration types
Domestic collaboration
Web of Science research areas
Orthopedics
Logo image