Journal article
Primary Structure and Function of an A Kinase Anchoring Protein Associated with Calcium Channels
Neuron (Cambridge, Mass.), v 20(5), pp 1017-1026
01 May 1998
PMID: 9620705
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Rapid, voltage-dependent potentiation of skeletal muscle L-type calcium channels requires phosphorylation by cAMP-dependent protein kinase (PKA) anchored via an A kinase anchoring protein (AKAP). Here we report the isolation, primary sequence determination, and functional characterization of AKAP15, a lipid-anchored protein of 81 amino acid residues with a single amphipathic helix that binds PKA. AKAP15 colocalizes with L-type calcium channels in transverse tubules and is associated with L-type calcium channels in transfected cells. A peptide fragment of AKAP15 encompassing the RII-binding domain blocks voltage-dependent potentiation. These results indicate that AKAP15 targets PKA to the calcium channel and plays a critical role in voltage-dependent potentiation and regulation of skeletal muscle contraction. The expression of AKAP15 in the brain and heart suggests that it may mediate rapid PKA regulation of L-type calcium channels in neurons and cardiac myocytes.
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Details
- Title
- Primary Structure and Function of an A Kinase Anchoring Protein Associated with Calcium Channels
- Creators
- Peter C Gray - University of WashingtonBarry D Johnson - University of WashingtonRuth E Westenbroek - University of WashingtonLara G Hays - University of WashingtonJohn R Yates - University of WashingtonTodd Scheuer - University of WashingtonWilliam A Catterall - University of WashingtonBrian J Murphy - University of Washington
- Publication Details
- Neuron (Cambridge, Mass.), v 20(5), pp 1017-1026
- Publisher
- Elsevier
- Number of pages
- 10
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000073864700020
- Scopus ID
- 2-s2.0-0032078084
- Other Identifier
- 991021903116704721
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- Web of Science research areas
- Neurosciences