Journal article
Primary structure and variability of partial sequences in nonstructural gene 5 region of hepatitis G virus
Zhong hua yi xue za zhi, v 77(3), pp 178-182
Mar 1997
PMID: 9596954
Abstract
OBJECTIVETo sequence partial genome of hepatitis G virus (HGV) in the sera of patients with different chronic liver disease and paid donors.METHODSTotal nucleic acids were extracted from the sera of 3 paid donors, 2 patients with liver cirrhosis, and 1 patient with non-A-E chronic hepatitis, then subjected to reverse transcriptase-nested-polymerase chain reaction (PCR) with primers derived from the putative nonstructural gene 5 (NS5). PCR products were directly sequenced after purification.RESULTSPartial HGV NS5 gene of 6 isolates exhibited 87.2% to 93.9% nucleotide sequence identities with 3 reported HGV isolates (GBV-C, PNF2161, R10291) while the nucleotide homologies among the 6 isolates were 90.1% to 93.8%. And 93.6% to 98.7 homolies were found at the deduced amino acid level when comparing the 6 isolates with 3 reported ones, while 93.61% to 98.40% amino acid identities were found among 6 isolates. In the region sequenced, there were 16 conserved proline residues and 8 conserved cysteine residues.CONCLUS
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Details
- Title
- Primary structure and variability of partial sequences in nonstructural gene 5 region of hepatitis G virus
- Creators
- J ChangL WeiQ Tao
- Publication Details
- Zhong hua yi xue za zhi, v 77(3), pp 178-182
- Resource Type
- Journal article
- Language
- Chinese
- Academic Unit
- Microbiology and Immunology
- Scopus ID
- 2-s2.0-0031083738
- Other Identifier
- 991020547434204721