Journal article
Probes for quantitating subpicogram amounts of HIV-1 RNA by molecular hybridization
Molecular and cellular probes, v 3(1), pp 73-86
1989
PMID: 2471923
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A set of probes was designed for the quantitation of HIV-1 RNA in infected cells by a molecular hybridization procedure called reversible target capture. Reversible target capture is analogous to sandwich hybridization, except that the link between hybrid complexes and the affinity support was reversible, allowing for repeated capture of hybrids on, and release from, fresh affinity support. Repeated cycles of capture resulted in a high degree of purification of hybrids from unreacted probe, thereby greatly reducing assay noise and increasing assay sensitivity. Probes against the HIV-1
pol gene were chosen because their target sequences were highly conserved among HIV-1 isolates, while being divergent enough to provide discrimination from other human T cell tropic viruses. Subpicogram quantities of HIV-1
pol gene RNA were measured with signal:noise ratios of over 10. Hybridization signal increased with increasing target RNA with a proportionality constant of 1.
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Details
- Title
- Probes for quantitating subpicogram amounts of HIV-1 RNA by molecular hybridization
- Creators
- David Gillespie - Hahnemann University HospitalJames Thompson - Hahnemann University HospitalRobert Solomon - Hahnemann University HospitalJervis Walter Thompson - Digital Media
- Publication Details
- Molecular and cellular probes, v 3(1), pp 73-86
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Digital Media
- Web of Science ID
- WOS:A1989U587000007
- Scopus ID
- 2-s2.0-0024638128
- Other Identifier
- 991019183972204721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- Biotechnology & Applied Microbiology
- Cell Biology