Journal article
Process formation in Sf9 cells induced by the expression of a microtubule-associated protein 2C-like construct
Proceedings of the National Academy of Sciences - PNAS, v 90(13), pp 6223-6227
01 Jul 1993
PMID: 8327502
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
To understand the roles of various microtubule-associated proteins (MAPs) in the development of axons and dendrites, we have expressed individual neuronal MAPs in normally rounded Sf9 host cells. We previously reported that expression of tau protein in these cells results in the elaboration of long processes containing dense bundles of microtubules (MTs). These bundles generally terminate in the hillock region of the cell body, and almost all of the MTs within the bundles are oriented with their plus ends distal to the cell body. Here we report the expression of a construct that approximates the MAP2C sequence and also induces the elaboration of processes with dense bundles of predominantly plus-end-distal MTs. Whereas tau generally results in a single process, there is a significantly greater tendency for the MAP2C-like construct to induce multiple processes. In contrast to the tau processes, the MT bundle in these processes extends far into the cell body. This latter observation suggests that MAP2C and tau have different effects on MT assembly and/or transport events in the cell. Although both of these MAPs can organize MTs that are competent to participate in process formation, the detailed organization of MTs induced by each of the two constructs is distinctive, and these differences may be relevant to axonal and dendritic differentiation.
Metrics
Details
- Title
- Process formation in Sf9 cells induced by the expression of a microtubule-associated protein 2C-like construct
- Creators
- N LeClerc - Harvard Medical School, Boston, MA 02115K S Kosik - Harvard Medical School, Boston, MA 02115N Cowan - Harvard Medical School, Boston, MA 02115T P Pienkowski - Harvard Medical School, Boston, MA 02115P W Baas - Harvard Medical School, Boston, MA 02115
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, v 90(13), pp 6223-6227
- Publisher
- PNAS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:A1993LM03500072
- Scopus ID
- 2-s2.0-0027318768
- Other Identifier
- 991014878264004721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology