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Journal article
Prolonged Morphine Exposure Induces Increased Firm Adhesion in an in Vitro Model of the Blood-Brain Barrier
International journal of molecular sciences, v 17(6), pp 916-916
01 Jun 2016
PMID: 27294916
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The blood-brain barrier (BBB) has been defined as a critically important protective barrier that is involved in providing essential biologic, physiologic, and immunologic separation between the central nervous system (CNS) and the periphery. Insults to the BBB can cause overall barrier damage or deregulation of the careful homeostasis maintained between the periphery and the CNS. These insults can, therefore, yield numerous phenotypes including increased overall permeability, interendothelial gap formation, alterations in cytokine and chemokine secretion, and accelerated cellular passage. The current studies expose the human brain microvascular endothelial cell line, hCMEC/D3, to prolonged morphine exposure and aim to uncover the mechanisms underlying alterations in barrier function in vitro. These studies show alterations in the mRNA and protein levels of the cellular adhesion molecules (CAMs) intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and activated leukocyte cell adhesion molecule that correlate with an increased firm adhesion of the CD3+ subpopulation of peripheral blood mononuclear cells (PBMCs). Overall, these studies suggest that prolonged morphine exposure may result in increased cell migration into the CNS, which may accelerate pathological processes in many diseases that involve the BBB.
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Details
- Title
- Prolonged Morphine Exposure Induces Increased Firm Adhesion in an in Vitro Model of the Blood-Brain Barrier
- Creators
- Marianne Strazza - Drexel UniversityVanessa Pirrone - Drexel UniversityBrian Wigdahl - Drexel UniversityWill Dampier - Drexel UniversityWei Lin - Peking UniversityRui Feng - University of PennsylvaniaMonique E. Maubert - Drexel UniversityBabette Weksler - Cornell UniversityIgnacio A. Romero - The Open UniversityPierre-Olivier Couraud - Institut Cochin, INSERM (Institut National de la Santé et de la Recherche Médicale), U1016, CNRS UMR8104, Université Paris Descartes, Sorbonne Paris Cité, Paris 75014, France.Michael R. Nonnemacher - Drexel University
- Publication Details
- International journal of molecular sciences, v 17(6), pp 916-916
- Publisher
- MDPI AG
- Number of pages
- 15
- Grant note
- 1P30 MH-092177-01A / Public Health Service, National Institutes of Health through grants from the National Institute of Mental Health Comprehensive NeuroAIDS Core Center (CNAC) Developmental Grant NS32092; NS46263 / National Institute of Neurological Disorders and Stroke; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) DA19807 / National Institute of Drug Abuse; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Drug Abuse (NIDA) P30MH092177 / NATIONAL INSTITUTE OF MENTAL HEALTH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Mental Health (NIMH) R01DA019807 / NATIONAL INSTITUTE ON DRUG ABUSE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Drug Abuse (NIDA); European Commission 5T32MH079785 / Ruth L. Kirschstein National Research Service Award; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01NS046263 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000378799300129
- Scopus ID
- 2-s2.0-84973526718
- Other Identifier
- 991019167714604721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary