Journal article
Prolonged exposure to the candidate microbicide C31G differentially reduces cellular sensitivity to agent re-exposure
Biomedicine & pharmacotherapy, v 59(8), pp 460-468
2005
PMID: 16154719
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Comparative assays of in vitro cytotoxicity using nonoxynol-9 (N-9) and the candidate microbicides C31G and sodium dodecyl sulfate (SDS) demonstrated that these agents, which are, respectively, characterized as nonionic, amphoteric, and anionic surfactants, differed in their concentration-dependent effects on cell viability, especially after prolonged exposure. We hypothesized that differences in cellular sensitivity may have been due, in part, to cellular changes induced by long-term exposure to each agent. To examine this possibility, HeLa cells were exposed to N-9, C31G, or SDS for extended periods of time and subsequently reassessed for sensitivity to each of these agents. Following 10 continuous days of C31G exposure, HeLa cells were less sensitive to a subsequent C31G exposure compared to cells that had not undergone long-term C31G treatment. Interestingly, long-term C31G exposure also changed subsequent sensitivity to N-9 but not SDS. In contrast, prolonged exposure to either N-9 or SDS did not reduce sensitivity to re-exposure. The effect of long-term C31G exposure was both concentration-dependent and transient, as treated cells reverted to pre-exposure sensitivity in a time-dependent manner following the cessation of C31G exposure. Lipid analyses of cells exposed to C31G for extended durations revealed altered phospholipid profiles relative to C31G-naïve cells. Experiments examining the individual components of C31G demonstrated the involvement of the amine oxide moiety in reductions in cellular sensitivity. These studies, which provide new information concerning the cytotoxicity of surfactant microbicides, suggest that cervicovaginal epithelial cells may have greater in vivo tolerance for products containing C31G through unique interactions between C31G and components of the cellular membranes.
Metrics
Details
- Title
- Prolonged exposure to the candidate microbicide C31G differentially reduces cellular sensitivity to agent re-exposure
- Creators
- Bradley J Catalone - Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USAMary Lee Ferguson - Department of Microbiology and Immunology (G44), Center for Sexually Transmitted Disease, Center for Molecular Therapeutics, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USAShendra R Miller - Department of Microbiology and Immunology (G44), Center for Sexually Transmitted Disease, Center for Molecular Therapeutics, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USADan Malamud - Department of Biochemistry, University of Pennsylvania School of Dental Medicine, Philadelphia, PA 19104, USATina Kish-Catalone - Department of Microbiology and Immunology (G44), Center for Sexually Transmitted Disease, Center for Molecular Therapeutics, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USANina J Thakkar - Department of Microbiology and Immunology (G44), Center for Sexually Transmitted Disease, Center for Molecular Therapeutics, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USAFred C Krebs - Department of Microbiology and Immunology (G44), Center for Sexually Transmitted Disease, Center for Molecular Therapeutics, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USAMary K Howett - Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104, USABrian Wigdahl - Department of Microbiology and Immunology (G44), Center for Sexually Transmitted Disease, Center for Molecular Therapeutics, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USA
- Publication Details
- Biomedicine & pharmacotherapy, v 59(8), pp 460-468
- Publisher
- Elsevier SAS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000232464500007
- Scopus ID
- 2-s2.0-25644448247
- Other Identifier
- 991014877710004721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Medicine, Research & Experimental
- Pharmacology & Pharmacy