Journal article
Propagation and senescence of human marrow stromal cells in culture: a simple colony‐forming assay identifies samples with the greatest potential to propagate and differentiate
British journal of haematology, v 107(2), pp 275-281
Nov 1999
PMID: 10583212
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Marrow stromal cells (MSCs) were isolated from bone marrow obtained by aspirates of the iliac crest of normal volunteers. The cells were isolated by their adherence to plastic and then passed in culture. Some of the samples expanded through over 15 cell doublings from the time frozen stocks were prepared. Others ceased replicating after about four cell doublings. The replicative potential of the cells in culture was best predicted by a simple colony‐forming assay in which samples from early passages were plated at low densities of about 10 cells per cm2. Samples with high colony‐forming efficiency exhibited the greatest replicative potential. The colonies obtained by plating early passage cells at low density varied in size and morphology. The large colonies readily differentiated into osteoblasts and adipocytes when incubated in the appropriate medium. As samples were expanded in culture and approached senescence, they retained their ability to differentiate into osteoblasts. However, the cells failed to differentiate into adipocytes. The loss of multipotentiality following serial passage in culture may have important implications for the use of expanded MSCs for cell and gene therapy.
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Details
- Title
- Propagation and senescence of human marrow stromal cells in culture: a simple colony‐forming assay identifies samples with the greatest potential to propagate and differentiate
- Creators
- Carla M. DiGirolamo - Drexel UniversityDavid Stokes - Center for Gene Therapy, and Department of Radiation Oncology, MCP Hahnemann University, Philadelphia, Pennsylvania, U.S.A.David Colter - Center for Gene Therapy, and Department of Radiation Oncology, MCP Hahnemann University, Philadelphia, Pennsylvania, U.S.A.Donald G. Phinney - Center for Gene Therapy, and Department of Radiation Oncology, MCP Hahnemann University, Philadelphia, Pennsylvania, U.S.A.Reiner Class - Gene (China)Darwin J. Prockop - Center for Gene Therapy, and Department of Radiation Oncology, MCP Hahnemann University, Philadelphia, Pennsylvania, U.S.A.
- Publication Details
- British journal of haematology, v 107(2), pp 275-281
- Publisher
- Blackwell Science Ltd
- Number of pages
- 7
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Radiation Oncology (and Nuclear Medicine)
- Web of Science ID
- WOS:000083703200008
- Scopus ID
- 2-s2.0-0032748173
- Other Identifier
- 991019168082204721
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- Web of Science research areas
- Hematology