Journal article
Proper Sterol Distribution Is Required for Candida albicans Hyphal Formation and Virulence
G3 : genes - genomes - genetics, v 6(11), pp 3455-3465
01 Nov 2016
PMID: 27587298
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
is an opportunistic fungus responsible for the majority of systemic fungal infections. Multiple factors contribute to
pathogenicity.
strains lacking CaArv1 are avirulent. Arv1 has a conserved Arv1 homology domain (AHD) that has a zinc-binding domain containing two cysteine clusters. Here, we explored the role of the CaAHD and zinc-binding motif in CaArv1-dependent virulence. Overall, we found that the CaAHD was necessary but not sufficient for cells to be virulent, whereas the zinc-binding domain was essential, as
cells expressing the full-length zinc-binding domain mutants, Caarv1
and Caarv1
, were avirulent. Phenotypically, we found a direct correlation between the avirulence of
,
,
, and
cells and defects in bud site selection, septa formation and localization, and hyphal formation and elongation. Importantly, all avirulent mutant strains lacked the ability to maintain proper sterol distribution. Overall, our results have established the importance of the AHD and zinc-binding domain in fungal invasion, and have correlated an avirulent phenotype with the inability to maintain proper sterol distribution.
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Details
- Title
- Proper Sterol Distribution Is Required for Candida albicans Hyphal Formation and Virulence
- Creators
- Paula McCourt - AnsysHsing-Yin Liu - AnsysJosie E Parker - Swansea UniversityChristina Gallo-Ebert - AnsysMelissa Donigan - AnsysAdam Bata - AnsysCaroline Giordano - AnsysSteven L Kelly - Swansea UniversityJoseph T Nickels, Jr - Ansys
- Publication Details
- G3 : genes - genomes - genetics, v 6(11), pp 3455-3465
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000388678000002
- Scopus ID
- 2-s2.0-84996602300
- Other Identifier
- 991021229992704721
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- Collaboration types
- International collaboration
- Web of Science research areas
- Genetics & Heredity