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Journal article
Protease-degradable electrospun fibrous hydrogels
Nature communications, v 6(1), pp 6639-6639
23 Mar 2015
PMID: 25799370
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Electrospun nanofibres are promising in biomedical applications to replicate features of the natural extracellular matrix (ECM). However, nearly all electrospun scaffolds are either non-degradable or degrade hydrolytically, whereas natural ECM degrades proteolytically, often through matrix metalloproteinases. Here we synthesize reactive macromers that contain protease-cleavable and fluorescent peptides and are able to form both isotropic hydrogels and electrospun fibrous hydrogels through a photoinitiated polymerization. These biomimetic scaffolds are susceptible to protease-mediated cleavage in vitro in a protease dose-dependent manner and in vivo in a subcutaneous mouse model using transdermal fluorescent imaging to monitor degradation. Importantly, materials containing an alternate and non-protease-cleavable peptide sequence are stable in both in vitro and in vivo settings. To illustrate the specificity in degradation, scaffolds with mixed fibre populations support selective fibre degradation based on individual fibre degradability. Overall, this represents a novel biomimetic approach to generate protease-sensitive fibrous scaffolds for biomedical applications.
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Details
- Title
- Protease-degradable electrospun fibrous hydrogels
- Creators
- Ryan J Wade - University of PennsylvaniaEthan J Bassin - University of PennsylvaniaChristopher B Rodell - University of PennsylvaniaJason A Burdick - University of Pennsylvania
- Publication Details
- Nature communications, v 6(1), pp 6639-6639
- Publisher
- Springer Nature
- Grant note
- R01 HL107938 / NHLBI NIH HHS R01 AR056624 / NIAMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000353040200001
- Scopus ID
- 2-s2.0-84925357679
- Other Identifier
- 991019176637804721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Materials Science, Biomaterials