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Protecting Myocardial Creatine Kinase Activity during Reperfusion Improves Bioenergetics and Contractile Function
Journal article   Peer reviewed

Protecting Myocardial Creatine Kinase Activity during Reperfusion Improves Bioenergetics and Contractile Function

Lewis J. Kaplan, Haywood Blum, Anirban Banerjee and Glenn J.R. Whitman
The Journal of surgical research, v 54(4), pp 311-315
1993
DOI: https://doi.org/10.1006/jsre.1993.1049
PMID: 8331924
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

The exact mechanisms underlying reperfusion (RP) injury are unclear but are thought to involve toxic oxygen metabolites such as H 2O 2. The purpose of our study was to determine whether increasing endogenous catalase (CAT) stores would protect creatine kinase (CK) activity and improve bioenergetics and mechanical function during RP. Rats ( n = 6/group) were pretreated with myristic acid (MA) or nothing 6 to 12 hr prior to cardiectomy. Hearts were Langendorff perfused and developed pressure (DP) was monitored during 25 min of 37°C ischemia and 10 or 40 min of RP. CK activity was determined at baseline, end ischemia, and 40 min RP. CAT activity and H 2O 2 production was assayed at baseline, end ischemia, and 10 min RP. 31P NMR spectra were continuously acquired to determine ATP and phosphocreatine (PCr) concentrations. MA-pretreated hearts demonstrated elevated CAT stores (121 ± 4%, P < 0.05). No H 2O 2 was produced during ischemia, and both groups generated significant but equal amounts of H 2O 2 at RP 10 ( P < 0.001 vs preischemia (PI), P = NS between groups). By RP 40, MA-pretreated hearts recovered more DP than did control hearts (75 ± 5% of PI vs 35 ± 4% of PI, P < 0.001) and retained more CK activity as well (66 ± 4% vs 52 ± 2%, P < .05). PCr/ATP ratios of control hearts were abnormally elevated above baseline and that of MA hearts during RP (2.4 ± 0.1 vs 1.85 ± 0.08, P < 0.05 at RP 40). We conclude that: (1) only RP generates H 2O 2, (2) CK inactivation occurs during RP, (3) MA augments endogenous CAT and protects CK during RP, and (4) enhanced CK activity results in improved mechanical and bioenergetic recovery.

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15 citations in Web of Science
12 citations in Scopus

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#3 Good Health and Well-Being

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Collaboration types
Domestic collaboration
Web of Science research areas
Surgery
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