Journal article
Protection against Plasmodium chabaudi Malaria Induced by Immunization with Apical Membrane Antigen 1 and Merozoite Surface Protein 1 in the Absence of Gamma Interferon or Interleukin-4
Infection and immunity, v 72(10), pp 5605-5612
Oct 2004
PMID: 15385457
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Strategies to optimize formulations of multisubunit malaria vaccines require a basic knowledge of underlying protective immune mechanisms induced by each vaccine component. In the present study, we evaluated the contribution of antibody-mediated and cell-mediated immune mechanisms to the protection induced by immunization with two blood-stage malaria vaccine candidate antigens, apical membrane antigen 1 (AMA-1) and merozoite surface protein 1 (MSP-1). Immunologically intact or selected immunologic knockout mice were immunized with purified recombinant
Plasmodium chabaudi
AMA-1 (PcAMA-1) and/or the 42-kDa C-terminal processing fragment of
P. chabaudi
MSP-1 (MSP-1
42
). The efficacy of immunization in each animal model was measured as protection against blood-stage
P. chabaudi
malaria. Immunization of B-cell-deficient J
H
−/−
mice indicated that PcAMA-1 vaccine-induced immunity is largely antibody dependent. In contrast, J
H
−/−
mice immunized with PcMSP-1
42
were partially protected against
P. chabaudi
malaria, indicating a role for protective antibody-dependent and antibody-independent mechanisms of immunity. The involvement of γδ T cells in vaccine-induced PcAMA-1 and/or PcMSP-1
42
protection was minor. Analysis of the isotypic profile of antigen-specific antibodies induced by immunization of immunologically intact mice revealed a dominant IgG1 response. However, neither interleukin-4 and the production of IgG1 antibodies nor gamma interferon and the production of IgG2a/c antibodies were essential for PcAMA-1 and/or PcMSP-1
42
vaccine-induced protection. Therefore, for protective antibody-mediated immunity, vaccine adjuvants and delivery systems for AMA-1- and MSP-1-based vaccines can be selected for their ability to maximize responses irrespective of IgG isotype or any Th1 versus Th2 bias in the CD4
+
-T-cell response.
Metrics
Details
- Title
- Protection against Plasmodium chabaudi Malaria Induced by Immunization with Apical Membrane Antigen 1 and Merozoite Surface Protein 1 in the Absence of Gamma Interferon or Interleukin-4
- Creators
- James M Burns - Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PennsylvaniaPatrick R Flaherty - Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PennsylvaniaPayal Nanavati - Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PennsylvaniaWilliam P Weidanz - Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania
- Publication Details
- Infection and immunity, v 72(10), pp 5605-5612
- Publisher
- American Society for Microbiology
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000224134000008
- Scopus ID
- 2-s2.0-4644359502
- Other Identifier
- 991014878409404721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology
- Infectious Diseases