Files and links (1)
Published, Version of Record (VoR)Open Access (License Unspecified), Open
Journal article
Protection against simian/human immunodeficiency virus (SHIV) 89.6P in macaques after coimmunization with SHIV antigen and IL-15 plasmid
Proceedings of the National Academy of Sciences - PNAS, v 104(47), pp 18648-18653
20 Nov 2007
PMID: 18000037
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The cell-mediated immune profile induced by a recombinant DNA vaccine was assessed in the simian/HIV (SHIV) and macaque model. The vaccine strategy included coimmunization of a DNA-based vaccine alone or in combination with an optimized plasmid encoding macaque IL-15 (pmacIL-15). We observed strong induction of vaccine-specific IFN-γ-producing CD8
+
and CD4
+
effector T cells in the vaccination groups. Animals were subsequently challenged with 89.6p. The vaccine groups were protected from ongoing infection, and the IL-15 covaccinated group showed a more rapidly controlled infection than the group treated with DNA vaccine alone. Lymphocytes isolated from the group covaccinated with pmacIL-15 had higher cellular proliferative responses than lymphocytes isolated from the macaques that received SHIV DNA alone. Vaccine antigen activation of lymphocytes was also studied for a series of immunological molecules. Although mRNA for IFN-γ was up-regulated after antigen stimulation, the inflammatory molecules IL-8 and MMP-9 were down-regulated. These observed immune profiles are potentially reflective of the ability of the different groups to control SHIV replication. This study demonstrates that an optimized IL-15 immune adjuvant delivered with a DNA vaccine can impact the cellular immune profile in nonhuman primates and lead to enhanced suppression of viral replication.
Metrics
Details
- Title
- Protection against simian/human immunodeficiency virus (SHIV) 89.6P in macaques after coimmunization with SHIV antigen and IL-15 plasmid
- Creators
- Jean D. Boyer - University of PennsylvaniaTara M. Robinson - University of PennsylvaniaMichele A. Kutzler - University of PennsylvaniaGordon Vansant - Althea TechnologiesDavid A. Hokey - University of PennsylvaniaSanjeev Kumar - University of PennsylvaniaRose Parkinson - University of PennsylvaniaLing Wu - University of PennsylvaniaManinder K. Sidhu - Vaccine Discovery, Wyeth, Pearl River, NY 10965George N. Pavlakis - National Institutes of HealthBarbara K. Felber - National Institutes of HealthCharles Brown - National Institutes of HealthPeter Silvera - Southern Research InstituteMark G. LewisJoseph Monforte - Althea TechnologiesThomas A. Waldmann - National Institutes of HealthJohn Eldridge - Institut de Recherche VaccinaleDavid B. Weiner - University of Pennsylvania
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, v 104(47), pp 18648-18653
- Publisher
- National Academy of Sciences
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Infectious Diseases (and HIV Medicine)
- Web of Science ID
- WOS:000251292500055
- Scopus ID
- 2-s2.0-36749042670
- Other Identifier
- 991020550347704721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Immunology