Journal article
Protective Immunization with a Novel Membrane Protein of Plasmodium yoelii-Infected Erythrocytes
Infection and immunity, v 67(2), pp 675-680
Feb 1999
PMID: 9916076
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Immunization with a particulate fraction of blood-stage antigens was shown previously to protect mice against
Plasmodium yoelii
malaria. To identify antigens inducing the protective response, sera from immunized mice were used to screen a
P. yoelii
cDNA expression library. Sequence analysis of one 2.6-kb cDNA clone indicated that the identified gene,
pypag-1
, encoded a novel plasmodial antigen. Two nonoverlapping regions of
pypag-1
were expressed in
Escherichia coli
. The first recombinant antigen, pAg-1N, contained the N-terminal 337 residues, which included a putative transmembrane domain and a region relatively rich in tryptophan residues. The second recombinant antigen, pAg-1C, contained the remaining C-terminal 211 residues, which included 31 copies of a 5-amino-acid degenerative repeat. Immunoblot studies using rabbit antiserum raised against recombinant pAg-1N showed that the native pypAg-1 protein migrated at approximately 98 kDa, considerably slower than its predicted molecular mass of 66 kDa. Immunofluorescence studies localized the expression of the native pypAg-1 protein both to the cytoplasm and at the surface of
P. yoelii
-infected erythrocytes. Immunization with either pAg-1N or pAg-1C induced a four- to sevenfold reduction in
P. yoelii
blood-stage parasitemia. As such, pypAg-1 appears to contain at least two distinct protective epitopes. To our knowledge, this is the first characterization of a protective antigen of
P. yoelii
that is associated with the erythrocyte membrane.
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Details
- Title
- Protective Immunization with a Novel Membrane Protein of Plasmodium yoelii-Infected Erythrocytes
- Creators
- James M Burns - Department of Microbiology, Meharry Medical College, Nashville, Tennessee 37208Eric K Adeeku - Department of Microbiology, Meharry Medical College, Nashville, Tennessee 37208Patricia D Dunn - Department of Microbiology, Meharry Medical College, Nashville, Tennessee 37208
- Publication Details
- Infection and immunity, v 67(2), pp 675-680
- Publisher
- American Society for Microbiology
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000078251400030
- Scopus ID
- 2-s2.0-0032964318
- Other Identifier
- 991014877851804721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Immunology
- Infectious Diseases