Journal article
Protective immunity against Plasmodium yoelii malaria induced by immunization with particulate blood-stage antigens
Infection and immunity, v 65(8), pp 3138-3145
Aug 1997
PMID: 9234766
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The Plasmodium yoelii murine model was used to test several combinations of blood-stage antigens and adjuvants for the ability to induce immunity to blood-stage malaria. Upon fractionation of whole blood-stage antigen into soluble and insoluble components, only the particulate antigens (pAg) induced protective immune responses. Of a number of adjuvants tested, Quil A was the most effective. Immunization with pAg plus Quil A induced solid protection against nonlethal and lethal P. yoelii challenge infection. Analysis of cytokine production revealed mRNA for Th1-type cytokines (interleukin 2 [IL-2] and gamma interferon) as well as Th2-type cytokines (IL-4 and IL-10) in the spleens of both protected and susceptible animals. The data suggested that the protective pAg response was associated with the earlier production of cytokine mRNA with a Th2 phenotype somewhat favored. Immunization of B-cell-deficient JHD mice indicated that the protection against P. yoelii induced by pAg immunization was B cell dependent. Although immunization with pAg plus Quil A increased the levels of antigen-specific antibodies of all four immunoglobulin G (IgG) isotypes, protection correlated most closely with the presence of IgG1 and IgG2b antibodies. Sera from pAg-plus-Quil A-immunized animals recognized only a limited subset of six to eight distinct P. yoelii antigens, primarily associated with the pAg fraction. These results provide the basis for the identification and characterization of potential vaccine antigens, selected solely for their ability to immunize against blood-stage malaria.
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Details
- Title
- Protective immunity against Plasmodium yoelii malaria induced by immunization with particulate blood-stage antigens
- Creators
- J M Burns, Jr - Department of Microbiology, Meharry Medical College, Nashville, Tennessee 37208, USAP D DunnD M Russo
- Publication Details
- Infection and immunity, v 65(8), pp 3138-3145
- Publisher
- American Society for Microbiology (ASM); United States
- Grant note
- AI335661 / NIAID NIH HHS G12RR03032 / NCRR NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- MD (Doctor of Medicine) Program; Microbiology and Immunology
- Web of Science ID
- WOS:A1997XM71400022
- Scopus ID
- 2-s2.0-0030878453
- Other Identifier
- 991014878421604721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Immunology
- Infectious Diseases