Journal article
Protein response of insect cells to bioreactor environmental stresses
Journal of biotechnology, v 118(3), pp 278-289
2005
PMID: 16002170
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Protein expression of
Spodoptera frugiperda (Sf9) insect cells was characterized upon exposure to environmental stresses typically present in bioreactors including heat shock, oxygen deprivation, shear stress, change of pH, and salinity or ethanol shock. This study fills the void in knowledge as to how bioreactor hydrodynamics, anoxia, small changes in pH as well as salinity alterations due to pH control or exposure to ethanol used in asepsis treatments affect protein expression in Sf9 cells. Heat shock at 43
°C induced proteins at 83
kDa, 68–78
kDa and six small heat shock proteins (hsps) at 23–15.5
kDa. Anaerobic conditions in CO
2 atmosphere reduced significantly the normal protein synthesis and induced a small subset of heat shock proteins at 70
kDa. Oxygen deprivation in nitrogen atmosphere transiently induces the 70
kDa proteins and had minor effects on the normal protein synthesis. Exposure to increased salinity or ethanol concentration failed to trigger the stress response, but may extensively inhibit the induction of normal proteins even though there was a negligible change in cell viability. Shear stress that had a major reducing effect on cell viability did not change the protein synthesis profile of Sf9 cells. Both long and short term exposures to small pH changes had negligible effects on protein synthesis.
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Details
- Title
- Protein response of insect cells to bioreactor environmental stresses
- Creators
- Anne Huhtala - Department of Chemical Technology, Helsinki University of Technology, P.O. Box 6100, FIN-02150 HUT, FinlandPekka Linko - Department of Chemical Technology, Helsinki University of Technology, P.O. Box 6100, FIN-02150 HUT, FinlandRaj Mutharasan - Department of Chemical and Biological Engineering, Drexel University, 32nd and Chestnut Streets, Philadelphia, PA 19104, USA
- Publication Details
- Journal of biotechnology, v 118(3), pp 278-289
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000231124200006
- Scopus ID
- 2-s2.0-22544476835
- Other Identifier
- 991014877791304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology