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Proteins, recognition networks and developing interfaces for macromolecular biosensing
Journal article

Proteins, recognition networks and developing interfaces for macromolecular biosensing

Mauro Sergi, John Zurawski, Simon Cocklin and Irwin Chaiken
Journal of molecular recognition, v 17(3)
May 2004
PMID: 15137030

Abstract

Protein Structure, Tertiary Amino Acid Sequence Cell Line Humans Molecular Sequence Data Proteins - analysis Phosphatidylcholines - metabolism Amino Acid Motifs Protein Interaction Mapping Receptors, CCR5 - metabolism Animals Proteins - metabolism Dogs Biosensing Techniques - methods Lipid Bilayers - chemistry Lipid Bilayers - metabolism Proteins - chemistry Binding Sites
Genomics and proteomics discovery is leading to the identification of all proteins and to the opportunity, and challenge, to reveal the protein recognition networks that drive virtually all biological processes. Over the past decade, biosensors have emerged as a key technology for detection and analysis of biomolecular interactions. An important limitation in developing such biosensors is that the focus has been mainly on sensor platforms, the transducing hardware that converts interaction signals into recorded data, without adequately considering the role of molecular interfaces, the elements of sensors that interact with analytes to produce signals. We have investigated this alternative focus by identifying and, where necessary, designing molecular interfaces that will more effectively drive new biosensor development and utilization in biomedical and biotechnological investigations. Here we describe our recent studies of coiled coil and lipid bilayer interfaces and the potential to use these to expand sensing technologies for multiplexed target detection and analysis in increasingly biologically relevant membrane like environments.

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Web of Science research areas
Biochemistry & Molecular Biology
Biophysics
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