Journal article
Proximity labeling expansion microscopy (PL-ExM) evaluates interactome labeling techniques
Journal of materials chemistry. B, Materials for biology and medicine, v 12(34), pp 8335-8348
28 Aug 2024
PMID: 39105364
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Understanding protein-protein interactions (PPIs) through proximity labeling has revolutionized our comprehension of cellular mechanisms and pathology. Various proximity labeling techniques, such as HRP, APEX, BioID, TurboID, and mu Map, have been widely used to biotinylate PPIs or organelles for proteomic profiling. However, the variability in labeling precision and efficiency of these techniques often results in limited reproducibility in proteomic detection. We address this persistent challenge by introducing proximity labeling expansion microscopy (PL-ExM), a super-resolution imaging technique that combines expansion microscopy with proximity labeling techniques. PL-ExM enabled up to 17 nm resolution with microscopes widely available, providing visual comparison of the labeling precision, efficiency, and false positives of different proximity labeling methods. Our mass spectrometry proteomic results confirmed that PL-ExM imaging is reliable in guiding the selection of proximity labeling techniques and interpreting the proteomic results with new spatial information.
Proximity labeling expansion microscopy (PL-ExM) visualizes superresolution structures of interactome on widely accessible light microscopes, enabling the assessment of the precision and efficiency of proximity labeling techniques.
Metrics
6 Record Views
Details
- Title
- Proximity labeling expansion microscopy (PL-ExM) evaluates interactome labeling techniques
- Creators
- Sohyeon Park - University of California, IrvineXiaorong Wang - University of California, IrvineYajin Mo - University of California, IrvineSicheng Zhang - University of California, IrvineXiangpeng Li - University of California, San FranciscoKatie C. Fong - University of California, IrvineClinton Yu - University of California, IrvineArthur A. Tran - University of California, San FranciscoLorenzo Scipioni - Fluor (United States)Zhipeng Dai - University of California, San FranciscoXiao Huang - Drexel UniversityLan Huang - University of California, IrvineXiaoyu Shi (Corresponding Author) - University of California, Irvine
- Publication Details
- Journal of materials chemistry. B, Materials for biology and medicine, v 12(34), pp 8335-8348
- Publisher
- Royal Society of Chemistry
- Number of pages
- 15
- Grant note
- DP2GM150017 / NIH Director's New Innovator Award; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA CZI Advancing Imaging through Collaborative Projects award Chan Zuckerberg Initiative (CZI) Visual Proteomics Imaging Award R35GM145249 / NIH Maximizing Investigators' Research Award; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA 594598 / Simons Foundation R00GM126136 / NIH Pathway to Independence Award; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA DMS1763272 / NSF; National Science Foundation (NSF) 2341058 / NSF Faculty Early Career Development (CAREER) Award; National Science Foundation (NSF)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:001284177700001
- Scopus ID
- 2-s2.0-85200921755
- Other Identifier
- 991022130635604721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Materials Science, Biomaterials