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Purification of Arp2/3 complex from Saccharomyces cerevisiae
Journal article   Open access

Purification of Arp2/3 complex from Saccharomyces cerevisiae

Lynda K. Doolittle, Michael K. Rosen and Shae B. Padrick
Methods in molecular biology (Clifton, N.J.), v 1046, pp 251-271
01 Jan 2013
DOI: https://doi.org/10.1007/978-1-62703-538-5_15
PMID: 23868593
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://europepmc.org/articles/pmc3995026View
Accepted (AM)Open Access (License Unspecified),  Open

Abstract

3 complex Actin nucleation factor Arp2 biochemical purification detailed protocol endogenous Saccharomyces cerevisiae source
Much of cellular control over actin dynamics comes through regulation of actin filament initiation. At the molecular level, this is accomplished through a collection of cellular protein machines, called actin nucleation factors, which position actin monomers to initiate a new actin filament. The Arp2/3 complex is a principal actin nucleation factor used throughout the eukaryotic family tree. The budding yeast Saccharomyces cerevisiae has proven to be not only an excellent genetic platform for the study of the Arp2/3 complex, but also an excellent source for the purification of endogenous Arp2/3 complex. Here we describe a protocol for the preparation of endogenous Arp2/3 complex from wild type Saccharomyces cerevisiae . This protocol produces material suitable for biochemical study, and yields milligram quantities of purified Arp2/3 complex.

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Collaboration types
Domestic collaboration
Web of Science research areas
Biochemical Research Methods
Biochemistry & Molecular Biology
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