Files and links (1)
Accepted (AM)Open Access (License Unspecified), Open
Journal article
Purification of Native Arp2/3 Complex from Bovine Thymus
Methods in molecular biology (Clifton, N.J.), v 1046
01 Jan 2013
PMID: 23868592
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The Arp2/3 complex is an actin filament nucleator involved in cell motility and vesicle trafficking. Owing to the role the complex plays in important and fundamental cell biological processes, the purified complex is used in biochemical assays, reconstituted motility assays and structural biology. As this is a eukaryotic complex assembled from seven polypeptides, the complex is purified from eukaryotic sources. Described here is a detailed method for purification of the complex from a mammalian tissue, bovine thymus.
Metrics
Details
- Title
- Purification of Native Arp2/3 Complex from Bovine Thymus
- Creators
- Lynda K. Doolittle - Howard Hughes Medical InstituteMichael K. Rosen - Howard Hughes Medical InstituteShae B. Padrick - Howard Hughes Medical Institute
- Publication Details
- Methods in molecular biology (Clifton, N.J.), v 1046
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000325641400015
- Scopus ID
- 2-s2.0-84934443478
- Other Identifier
- 1627035389; 9781627035385; 991020836344204721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology