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Putative GTPase Gtr1p genetically interacts with the RanGTPase cycle in Saccharomyces cerevisiae
Journal article   Peer reviewed

Putative GTPase Gtr1p genetically interacts with the RanGTPase cycle in Saccharomyces cerevisiae

N Nakashima, N Hayashi, E Noguchi and T Nishimoto
Journal of cell science, v 109 ( Pt 9)(9), pp 2311-2318
Sep 1996
DOI: https://doi.org/10.1242/jcs.109.9.2311
PMID: 8886981
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Temperature Saccharomyces cerevisiae - genetics Genes, Suppressor Nuclear Proteins - metabolism DNA Primers - genetics Fungal Proteins - genetics DNA-Binding Proteins - genetics Guanine Nucleotide Exchange Factors DNA-Binding Proteins - metabolism Saccharomyces cerevisiae - metabolism GTP Phosphohydrolases - metabolism GTP Phosphohydrolases - genetics Base Sequence Gene Deletion Genes, Fungal Mutation Nuclear Proteins - genetics ran GTP-Binding Protein Cell Cycle Proteins Fungal Proteins - metabolism
In order to identify a protein interacting with RCC1, a guanine nucleotide-exchange factor for the nuclear GTPase Ran, we isolated a series of cold-sensitive suppressors of mtr1-2, a temperature-sensitive mutant of the Saccharomyces cerevisiae RCC1 homologue. One of the isolated suppressor mutants was mutated in the putative GTPase Gtr1p, being designated as gtr1-11. It also suppressed other alleles of mtr1-2, srm1-1 and prp20-1 in contrast to overexpression of the S. cerevisiae Ran/TC4 homologue Gsp1p, previously reported to suppress prp20-1, but not mtr1-2 or srm1-1. Furthermore, gtr1-11 suppressed the rna1-1, temperature-sensitive mutant of the Gsp1p GTPase-activating protein, but not the srp1-31, temperature-sensitive mutant of the S. cerevisiae importin alpha homologue. mtr1-2, srm1-1 and prp20-1 were also suppressed by overexpression of the mutated Gtr1p, Gtr1-11p. In summary, Gtr1p that was localized in the cytoplasm by immunofluoresence staining was suggested to function as a negative regulator for the Ran/TC4 GTPase cycle.

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