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Journal article
Quantifying the Biomechanics of Conception: L-Selectin-Mediated Blastocyst Implantation Mechanics with Engineered “Trophospheres”
Tissue engineering. Part A, v 20(1-2), pp 189-196
01 Jan 2014
PMID: 23927766
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
An estimated 12% of women in the United States suffer from some form of infertility.
In vitro
fertilization (IVF) is the most common treatment for infertility encompassing over 99% of all assisted reproductive technologies. However, IVF has a low success rate. Live birth rates using IVF can range from 40% in women younger than 35 years to 4% in women older than 42 years. Costs for a successful IVF outcome can be upward of $61,000. The low success rate of IVF has been attributed to the inability of the blastocyst to implant to the uterus. Blastocyst implantation is initiated by L-selectin expressing cells, trophoblasts, binding to L-selectin ligands, primarily sialyl Lewis X (sLeX), on the uterine surface endometrium. Legal and ethical considerations have limited the research on human subjects and tissues, whereas animal models are costly or do not properly mimic human implantation biochemistry. In this work, we describe a cellular model system for quantifying L-selectin adhesion mechanics. L-selectin expression was confirmed in Jeg-3, JAR, and BeWo cell lines, with only Jeg-3 cells exhibiting surface expression. Jeg-3 cells were cultured into three-dimensional spheres, termed “trophospheres,” as a mimic to human blastocysts. Detachment assays using a custom-built parallel plate flow chamber show that trophospheres detach from sLeX functionalized slides with 2.75×10
−3
dyn of force and 7.5×10
−5
dyn-cm of torque. This work marks the first time a three-dimensional cell model has been utilized for quantifying L-selectin binding mechanics related to blastocyst implantation.
Metrics
Details
- Title
- Quantifying the Biomechanics of Conception: L-Selectin-Mediated Blastocyst Implantation Mechanics with Engineered “Trophospheres”
- Creators
- Robert W. Yucha - Drexel UniversityMonika Jost - Drexel UniversityDianne Rothstein - 3Prime Synthesis, Inc., Aston, PennsylvaniaNoreen Robertson - Drexel UniversityMichele S. Marcolongo - Drexel University
- Publication Details
- Tissue engineering. Part A, v 20(1-2), pp 189-196
- Publisher
- Mary Ann Liebert, Inc
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- SOM Dean - Research Administration; Intensive Medical Sciences (IMS)
- Web of Science ID
- WOS:000337280600018
- Scopus ID
- 2-s2.0-84891509890
- Other Identifier
- 991021448045104721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cell & Tissue Engineering
- Cell Biology
- Engineering, Biomedical
- Materials Science, Biomaterials