Journal article
Quantifying the l-arginine paradox in vivo
Microvascular research, v 71(1), pp 48-54
2006
PMID: 16316668
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
NO and PO
2 microelectrodes were used to quantify the effects of increased availability of
l-arginine in an exteriorized rat mesentery and small intestine microcirculatory preparation in
n = 16 rats. During short periods of elevated
l-arginine added to the superfusion bath, transient changes in perivascular NO or PO
2 were measured at 171 perivascular sites near intestinal arterioles and venules, simultaneously with tissue perfusion using laser Doppler flowmetry (LDF). Excess
l-arginine increased perivascular NO over twofold, by 411 ± 42 nM above the baseline of 329 ± 30 nM (
P < 0.0001), and increased tissue perfusion by 35.5 ± 7.5% (
P < 0.0001). No difference between arterioles and venules was observed in the magnitude or time course of the NO responses. Both increases and decreases in perivascular PO
2 were observed after excess
l-arginine, with a similar increase in tissue perfusion by 42.0 ± 12.3% (
P < 0.0001). Our NO measurements confirm that increased bioavailability of
l-arginine causes a significant increase in NO production throughout the microcirculation of this preparation, with increased tissue perfusion, and provides direct in vivo evidence for the
l-arginine paradox.
Metrics
Details
- Title
- Quantifying the l-arginine paradox in vivo
- Creators
- Nina Vukosavljevic - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, USADov Jaron - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, USAKenneth A Barbee - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, USADonald G Buerk - Department of Physiology, University of Pennsylvania, A207 Richards Building, 3700 Hamilton Walk, Philadelphia, PA 19104-6086, USA
- Publication Details
- Microvascular research, v 71(1), pp 48-54
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; [Retired Faculty]
- Web of Science ID
- WOS:000235435800006
- Scopus ID
- 2-s2.0-31744439701
- Other Identifier
- 991014878600604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Peripheral Vascular Disease