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Quantitative Proviral DNA and Antibody Levels in the Natural History of HTLV-I Infection
Journal article   Open access   Peer reviewed

Quantitative Proviral DNA and Antibody Levels in the Natural History of HTLV-I Infection

Angela Manns, Wendell J. Miley, Rainford J. Wilks, Owen St. C. Morgan, Barrie Hanchard, Gilian Wharfe, Beverly Cranston, Elizabeth Maloney, Seth L. Welles, William A. Blattner, …
The Journal of infectious diseases, v 180(5), pp 1487-1493
01 Nov 1999
PMID: 10515807
url
https://doi.org/10.1086/315088View
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze) Open

Abstract

The pathogenesis of human T-cell lymphotropic virus type I (HTLV-I) in adult T-cell leukemia/lymphoma (ATL) and HTLV-I associated myelopathy/tropical spastic paraparesis (HAM/TSP) is poorly understood. We prospectively followed up and evaluated the virologic correlates of infection in transfusion recipients after seroconversion, in asymptomatic carriers, and in ATL and HAM/TSP patients. Proviral DNA levels (copies/105 lymphocytes) were determined by real-time automated polymerase chain reaction and antibody titers by end-point dilution by use of an HTLV-I enzyme-linked immunoassay. In early infection, proviral load was initially elevated (median, 212 copies/105 lymphocytes at time 1) and later decreased (median, 99 copies at time 2, and 27 copies at time 3). Corresponding antibody titers were low at time 1 (1 : 2154), had significantly increased by time 2 (1 : 12312), and were stable by time 3 (1 : 4694). These viral markers were significantly lower in asymptomatic carriers than in HAM/TSP or ATL patients. Therefore, proviral load and antibody titers may be useful as predictive markers of disease among carriers.

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Industry collaboration
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Web of Science research areas
Immunology
Infectious Diseases
Microbiology
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